Engström P E, Sundin U, Lavö B, Norhagen G, Hällgren R, Smith C I, Hammarström L
Department of Clinical Immunology, Karolinska Institute, Huddinge Hospital, Sweden.
Immunology. 1992 Dec;77(4):604-8.
The class and subclass distribution of antibodies against gliadin in intestinal lavage fluid, saliva and serum was investigated in individuals with coeliac disease. Serum antibodies against gliadin were mainly or even exclusively of the IgA1 subclass. In intestinal lavage fluid and saliva, antibodies of both IgA1 and IgA2 subclasses were found. In patients with and without IgA deficiency, an IgG response was detected both in serum and intestinal lavage fluid with a predominance of IgG1 in selected patients. Specific IgG2, IgG3 and IgG4 antibodies were also detected in intestinal lavage fluid, while no specific IgG2, IgG3 or IgG4 antibodies were found in serum, suggesting a local production of specific IgG antibodies. In Western blot analysis, intestinal lavage fluid and serum IgA antibodies reacted against gliadin components with a MW between 33,000 and 42,000. Serum IgA1 antibodies directed against a gliadin component with a MW slightly higher than 42,000 were also observed. Specific IgG and IgM antibodies in both the secretion and serum against gliadin components with a MW between 33,000 and 42,000 were also detected. This study shows that mucosa-derived gliadin-specific IgA and IgG antibodies may be produced even when there is an absence of specific antibodies of the corresponding immunoglobulin subclass in serum. Furthermore, the specificity of serum and intestinal lavage fluid anti-gliadin IgA1 antibodies may differ.
对患有乳糜泻的个体，研究了肠道灌洗液、唾液和血清中抗麦醇溶蛋白抗体的类别和亚类分布。血清中抗麦醇溶蛋白抗体主要甚至完全是IgA1亚类。在肠道灌洗液和唾液中，发现了IgA1和IgA2亚类的抗体。在有和没有IgA缺乏的患者中，在血清和肠道灌洗液中均检测到IgG反应，在部分患者中以IgG1为主。在肠道灌洗液中还检测到特异性IgG2、IgG3和IgG4抗体，而在血清中未发现特异性IgG2、IgG3或IgG4抗体，提示特异性IgG抗体是局部产生的。在蛋白质印迹分析中，肠道灌洗液和血清中的IgA抗体与分子量在33,000至42,000之间的麦醇溶蛋白成分发生反应。还观察到血清中针对分子量略高于42,000的麦醇溶蛋白成分的IgA1抗体。在分泌物和血清中还检测到针对分子量在33,000至42,000之间的麦醇溶蛋白成分的特异性IgG和IgM抗体。这项研究表明，即使血清中缺乏相应免疫球蛋白亚类的特异性抗体，也可能产生黏膜来源的麦醇溶蛋白特异性IgA和IgG抗体。此外，血清和肠道灌洗液抗麦醇溶蛋白IgA1抗体的特异性可能不同。
