Zhang Feng, Xu Gaopo, Geng Longpo, Lu Xiaoyan, Yang Kunlong, Yuan Jun, Nie Xinyi, Zhuang Zhenhong, Wang Shihua
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Toxins (Basel). 2016 Jul 5;8(7):202. doi: 10.3390/toxins8070202.
This study focused on AflSkn7, which is a stress response regulator in the aflatoxin-producing Aspergillus flavus. The ΔAflSkn7 mutants exhibited partially defective conidial formation and a complete inability to generate sclerotia, indicating AflSkn7 affects A. flavus asexual and sexual development. The mutants tolerated osmotic stress but were partially susceptible to the effects of cell wall stress. Additionally, the ΔAflSkn7 mutants were especially sensitive to oxidative stress. These observations confirmed that AflSkn7 influences oxidative stress responses rather than osmotic stress responses. Additionally, AflSkn7 was observed to increase aflatoxin biosynthesis and seed infection rates. These results indicate AflSkn7 affects A. flavus morphological development, stress response, aflatoxin production, and pathogenicity. The results of this study may facilitate the development of new methods to manage A. flavus infections.
本研究聚焦于黄曲霉毒素产生菌黄曲霉中的应激反应调节因子AflSkn7。ΔAflSkn7突变体表现出部分缺陷的分生孢子形成，且完全无法产生菌核，这表明AflSkn7影响黄曲霉的无性和有性发育。这些突变体耐受渗透胁迫，但对细胞壁胁迫的影响部分敏感。此外，ΔAflSkn7突变体对氧化胁迫特别敏感。这些观察结果证实AflSkn7影响氧化胁迫反应而非渗透胁迫反应。此外，观察到AflSkn7会增加黄曲霉毒素的生物合成和种子感染率。这些结果表明AflSkn7影响黄曲霉的形态发育、应激反应、黄曲霉毒素产生和致病性。本研究结果可能有助于开发管理黄曲霉感染的新方法。
