School of Food Science and Biotechnology, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea.
Toxins (Basel). 2019 Sep 27;11(10):567. doi: 10.3390/toxins11100567.
The calcineurin pathway is an important signaling cascade for growth, sexual development, stress response, and pathogenicity in fungi. In this study, we investigated the function of CrzA, a key transcription factor of the calcineurin pathway, in an aflatoxin-producing fungus . To examine the role of the gene, deletion mutant strains in were constructed and their phenotypes, including fungal growth, spore formation, and sclerotial formation, were examined. Absence of results in decreased colony growth, the number of conidia, and sclerocia production. The -deficient mutant strains were more susceptible to osmotic pressure and cell wall stress than control or complemented strains. Moreover, deletion of results in a reduction in aflatoxin production. Taken together, these results demonstrate that CrzA is important for differentiation and mycotoxin production in .
钙调磷酸酶途径是真菌生长、性发育、应激反应和致病性的重要信号级联。在这项研究中，我们研究了钙调磷酸酶途径中的关键转录因子 CrzA 在产黄曲霉中的功能。为了研究该基因的作用，构建了缺失突变株，并检测了它们的表型，包括真菌生长、孢子形成和菌核形成。 缺失导致菌落生长、分生孢子数量和菌核形成减少。与对照或互补菌株相比， -缺陷突变株对渗透压和细胞壁应激更敏感。此外，缺失导致黄曲霉毒素产量降低。综上所述，这些结果表明 CrzA 对产黄曲霉的分化和产毒至关重要。
