Qu Wenchao, Robert Christelle A M, Erb Matthias, Hibbard Bruce E, Paven Maxim, Gleede Tassilo, Riehl Barbara, Kersting Lena, Cankaya Aylin S, Kunert Anna T, Xu Youwen, Schueller Michael J, Shea Colleen, Alexoff David, Lee So Jeong, Fowler Joanna S, Ferrieri Richard A
Department of Biology, Brookhaven National Laboratory, Upton, New York 11973 (W.Q., Y.X., M.J.S., C.S., D.A., S.J.L., J.S.F., R.A.F.);Biotic Interactions, Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Plant Genetics Research Unit, United States Department of Agriculture Agricultural Research Service, University of Missouri, Columbia, Missouri 65211 (B.E.H.); andInstitut für Kernchemie, Johannes Gutenberg-Universität, 55128 Mainz, Germany (M.P., T.G., B.R., L.K., A.S.C., A.T.K.).
Department of Biology, Brookhaven National Laboratory, Upton, New York 11973 (W.Q., Y.X., M.J.S., C.S., D.A., S.J.L., J.S.F., R.A.F.);Biotic Interactions, Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland (C.A.M.R., M.E.);Plant Genetics Research Unit, United States Department of Agriculture Agricultural Research Service, University of Missouri, Columbia, Missouri 65211 (B.E.H.); andInstitut für Kernchemie, Johannes Gutenberg-Universität, 55128 Mainz, Germany (M.P., T.G., B.R., L.K., A.S.C., A.T.K.)
Plant Physiol. 2016 Oct;172(2):776-788. doi: 10.1104/pp.16.00735. Epub 2016 Jul 12.
The western corn rootworm (WCR; Diabrotica virgifera virgifera LeConte) is a major pest of maize (Zea mays) that is well adapted to most crop management strategies. Breeding for tolerance is a promising alternative to combat WCR but is currently constrained by a lack of physiological understanding and phenotyping tools. We developed dynamic precision phenotyping approaches using C with positron emission tomography, root autoradiography, and radiometabolite flux analysis to understand maize tolerance to WCR Our results reveal that WCR attack induces specific patterns of lateral root growth that are associated with a shift in auxin biosynthesis from indole-3-pyruvic acid to indole-3-acetonitrile. WCR attack also increases transport of newly synthesized amino acids to the roots, including the accumulation of Gln. Finally, the regrowth zones of WCR-attacked roots show an increase in Gln turnover, which strongly correlates with the induction of indole-3-acetonitrile-dependent auxin biosynthesis. In summary, our findings identify local changes in the auxin biosynthesis flux network as a promising marker for induced WCR tolerance.
西部玉米根萤叶甲（WCR；Diabrotica virgifera virgifera LeConte）是玉米（Zea mays）的主要害虫，它能很好地适应大多数作物管理策略。培育耐受性是对抗WCR的一种有前景的替代方法，但目前受到生理理解和表型分析工具不足的限制。我们使用正电子发射断层扫描、根放射自显影和放射性代谢物通量分析开发了动态精确表型分析方法，以了解玉米对WCR的耐受性。我们的结果表明，WCR攻击会诱导侧根生长的特定模式，这与生长素生物合成从吲哚-3-丙酮酸向吲哚-3-乙腈的转变有关。WCR攻击还会增加新合成氨基酸向根部的运输，包括谷氨酰胺的积累。最后，受WCR攻击的根的再生区域显示谷氨酰胺周转率增加，这与吲哚-3-乙腈依赖性生长素生物合成的诱导密切相关。总之，我们的研究结果确定生长素生物合成通量网络中的局部变化是诱导WCR耐受性的一个有前景的标志物。
