Park Sooyeon, Jung Yong-Taek, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4002-4008. doi: 10.1099/ijsem.0.001301. Epub 2016 Jul 13.
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated KEM-4T, was isolated from water of an estuary environment on the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-4T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain KEM-4T fell within the clade comprising the type strains of species of the genus Altererythrobacter, clustering with the type strains of Altererythrobacter aestiaquae, Altererythrobacter gangjinensis and Altererythrobacter luteolus, showing 95.0-96.5 % sequence similarity. Sequences similarities to the type strains of the other species of the genus Altererythrobacter were 93.1-95.1 %. Strain KEM-4T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C17 : 1ω6c as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain KEM-4T was 59.5 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain KEM-4T is separated from species of the genus Altererythrobacter with validly published names. On the basis of the data presented, strain KEM-4T is considered to represent a novel species of the genus Altererythrobacter, for which the name Altererythrobacter confluentissp. nov. is proposed. The type strain is KEM-4T (=KCTC 52259T=NBRC 112305T).
从韩国黄海河口环境的水中分离出一株革兰氏染色阴性、需氧、不运动、卵圆形或杆状的细菌菌株，命名为KEM-4T，并对其进行了多相分类研究。菌株KEM-4T在pH 7.0-8.0、30 °C以及1.0-2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株KEM-4T属于包含交替赤杆菌属各物种模式菌株的进化分支，与嗜盐交替赤杆菌、康津交替赤杆菌和浅黄交替赤杆菌的模式菌株聚类，序列相似性为95.0-96.5 %。与交替赤杆菌属其他物种模式菌株的序列相似性为93.1-95.1 %。菌株KEM-4T以Q-10作为主要泛醌，以C18 : 1ω7c、总和特征3（C16 : 1ω6c和/或C16 : 1ω7c）以及C17 : 1ω6c作为主要脂肪酸。主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂。菌株KEM-4T的DNA G+C含量为59.5 mol%。不同的表型特征以及系统发育独特性表明，菌株KEM-4T与已有效发表名称的交替赤杆菌属物种不同。根据所提供的数据，菌株KEM-4T被认为代表交替赤杆菌属的一个新物种，为此提出新名称融合交替赤杆菌（Altererythrobacter confluentissp. nov.）。模式菌株为KEM-4T（=KCTC 52259T=NBRC 112305T）。
