Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 461-701, Korea.
Lab Chip. 2016 Aug 16;16(17):3251-9. doi: 10.1039/c6lc00751a.
Here, we introduce a simple and fast method for bonding a poly(dimethylsiloxane) (PDMS) silicone elastomer to different plastics. In this technique, surface modification and subsequent bonding processes are performed at room temperature. Furthermore, only one chemical is needed, and no surface oxidation step is necessary prior to bonding. This bonding method is particularly suitable for encapsulating biomolecules that are sensitive to external stimuli, such as heat or plasma treatment, and for embedding fracturable materials prior to the bonding step. Microchannel-fabricated PDMS was first oxidized by plasma treatment and reacted with aminosilane by forming strong siloxane bonds (Si-O-Si) at room temperature. Without the surface oxidation of the amine-terminated PDMS and plastic, the two heterogeneous substrates were brought into intimate physical contact and left at room temperature. Subsequently, aminolysis occurred, leading to the generation of a permanent seal via the formation of robust urethane bonds after only 5 min of assembling. Using this method, large-area (10 × 10 cm) bonding was successfully realized. The surface was characterized by contact angle measurements and X-ray photoelectron spectroscopy (XPS) analyses, and the bonding strength was analyzed by performing peel, delamination, leak, and burst tests. The bond strength of the PDMS-polycarbonate (PC) assembly was approximately 409 ± 6.6 kPa, and the assembly withstood the injection of a tremendous amount of liquid with the per-minute injection volume exceeding 2000 times its total internal volume. The thermal stability of the bonded microdevice was confirmed by performing a chamber-type multiplex polymerase chain reaction (PCR) of two major foodborne pathogens - Escherichia coli O157:H7 and Salmonella typhimurium - and assessing the possibility for on-site direct detection of PCR amplicons. This bonding method demonstrated high potential for the stable construction of closed microfluidic systems socketed with biomolecule-immobilized surfaces such as DNA, antibody, enzyme, peptide, and protein microarrays.
在这里，我们介绍了一种将聚二甲基硅氧烷（PDMS）硅橡胶与不同塑料粘合的简单快速方法。在该技术中，表面改性和随后的粘合过程在室温下进行。此外，仅需要一种化学物质，并且在粘合之前不需要进行表面氧化步骤。这种粘合方法特别适用于封装对外部刺激（如热或等离子体处理）敏感的生物分子，以及在粘合步骤之前嵌入易碎材料。首先通过等离子体处理氧化微通道制造的 PDMS，并通过在室温下形成强硅氧烷键（Si-O-Si）与氨硅烷反应。无需对胺封端的 PDMS 和塑料进行表面氧化，将两个异质基底紧密物理接触并留在室温下。随后，氨解发生，仅在组装 5 分钟后通过形成强的氨基甲酸酯键生成永久性密封。使用这种方法，成功实现了大面积（10×10cm）的键合。通过接触角测量和 X 射线光电子能谱（XPS）分析对表面进行了表征，并通过进行剥离、分层、泄漏和爆裂测试分析了粘合强度。PDMS-聚碳酸酯（PC）组件的粘合强度约为 409±6.6kPa，并且组件能够承受以每分钟超过其总体积 2000 倍的注射量注入的大量液体。通过对两种主要食源性病原体 - 大肠杆菌 O157：H7 和鼠伤寒沙门氏菌 - 进行室型多重聚合酶链反应（PCR），并评估对现场直接检测 PCR 扩增子的可能性，证实了键合微器件的热稳定性。这种粘合方法为稳定构建带有固定生物分子的表面（例如 DNA、抗体、酶、肽和蛋白质微阵列）的密闭微流控系统提供了很高的潜力。
