Miller M W, Azadniv M, Pettit S E, Church C C, Carstensen E L, Hoffman D
Department of Biophysics, School of Medicine and Dentistry, University of Rochester, NY 14642.
Ultrasound Med Biol. 1989;15(3):255-62. doi: 10.1016/0301-5629(89)90069-0.
This study was undertaken in an attempt to determine a physical mechanism of action for a recently published report of a small but statistically significant increase in sister chromatid exchanges (SCEs) in Chinese hamster ovary cells exposed to high-intensity pulsed ultrasound. The "positive" report's protocol involved a sizeable chance of ultrasound beam impingement on the side wall of the cell exposure chamber. Ten experiments per regimen were conducted; the regimens included exposures of (a) chamber center, (b) chamber wall, (c) nine grid sites, 0.5 mm between sites, and (d) nine grid sites, 1.5 mm between sites. The last was an exact replication of the conditions previously reported to induce the small SCE effect. The results did not support the postulate of an increase in SCEs with the ultrasound exposures.
本研究旨在确定一种物理作用机制，该机制与最近发表的一份报告相关，该报告指出，暴露于高强度脉冲超声的中国仓鼠卵巢细胞中，姐妹染色单体交换（SCE）出现了虽小但具有统计学意义的增加。“阳性”报告的实验方案存在超声束撞击细胞暴露室侧壁的较大可能性。每个方案进行了10次实验；这些方案包括暴露于（a）室中心，（b）室壁，（c）九个网格点，点间距为0.5毫米，以及（d）九个网格点，点间距为1.5毫米。最后一组是对先前报道的诱导小SCE效应的条件的精确复制。结果不支持超声暴露会导致SCE增加的假设。
