Sadaghian Mohammad, Amani Sasan, Jafari Rasool
Department of Pathobiology, School of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran.
Department of Veterinary Parasitology, Boroujerd Science and Research Branch, Islamic Azad University, Boroujerd, Iran.
J Parasit Dis. 2016 Jun;40(2):520-3. doi: 10.1007/s12639-014-0537-0. Epub 2014 Aug 31.
Toxoplasmosis is mostly asymptomatic infection in immunocompetent individuals while it can cause a severe infection in human fetus during pregnancy and immunocompromised patients. This study aimed to determine the prevalence of anti-Toxoplasma IgM and IgG seropositivity and potential risk factors of the infection in humans referred to Urmia City main diagnostic laboratories, Urmia, Iran. Totally 195 blood samples were collected from the individuals referred to main diagnostic laboratories of Urmia City, 2013. Serum concentration of anti-Toxoplasma IgG and IgM were determined using ELISA method. Demographic variables of the participants were collected by interviewing, which are including sex, age, occupation, educational and residential status, eating undercooked meat, consumption of raw vegetable and the method of washing raw vegetables. None of all 200 serum sample were anti-Toxoplasma IgM positive, but different concentrations of anti-Toxoplasma IgG were observed in 88 (45.12 %) of samples. The significant higher rate of anti-Toxoplasma IgG seropositivity were observed in people with soil related jobs (P = 0.005, OR = 2.266; 95 % CI 1.260, 4.078) and history of eating raw vegetables at restaurant (P = 0.036, OR = 1.985; 95 % CI 0.991, 3.978). Also anti-Toxoplasma IgG concentration mean was significantly higher in people who were commonly eaten raw vegetable at restaurants (P < 0.001, t = 7.918). The prevalence of chronic toxoplasmosis is considerably high while the acute infection is very low in the studied area. Having soil related jobs and eating raw vegetables at restaurants increases the risk of acquiring the infection.
弓形虫病在免疫功能正常的个体中大多为无症状感染，而在孕期可导致人类胎儿严重感染，在免疫功能低下的患者中也会引发严重感染。本研究旨在确定转诊至伊朗乌尔米耶市主要诊断实验室的人群中抗弓形虫IgM和IgG血清阳性率以及感染的潜在风险因素。2013年，从转诊至乌尔米耶市主要诊断实验室的个体中总共采集了195份血样。采用酶联免疫吸附测定（ELISA）法测定血清中抗弓形虫IgG和IgM的浓度。通过访谈收集参与者的人口统计学变量，包括性别、年龄、职业、教育程度和居住状况、食用未煮熟肉类、食用生蔬菜以及清洗生蔬菜的方式。所有200份血清样本中均无抗弓形虫IgM阳性，但在88份（45.12%）样本中观察到不同浓度的抗弓形虫IgG。从事与土壤相关工作的人群（P = 0.005，比值比[OR] = 2.266；95%置信区间[CI] 1.260，4.078）和有在餐馆食用生蔬菜史的人群（P = 0.036，OR = 1.985；95% CI 0.991，3.978）中抗弓形虫IgG血清阳性率显著更高。此外，经常在餐馆食用生蔬菜的人群中抗弓形虫IgG浓度均值显著更高（P < 0.001，t = 7.918）。在所研究地区，慢性弓形虫病的患病率相当高，而急性感染率很低。从事与土壤相关工作以及在餐馆食用生蔬菜会增加感染风险。
