Chen Mu-Xin, Chen Jia-Xu, Chen Shao-Hong, Huang Da-Na, Ai Lin, Zhang Ren-Li
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, National Health and Family Planning Commission, Shanghai 200025, PR China.
Shenzhen Center for Diseases Control and Prevention, Shenzhen, Guangdong Province 518055, PR China.
Korean J Parasitol. 2016 Jun;54(3):375-80. doi: 10.3347/kjp.2016.54.3.375. Epub 2016 Jun 30.
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
由于没有理想的诊断方法，广州管圆线虫病很难被诊断出来。血清学检测需要特定设备，在贫困地区并不总是能够获得，而且耗时较长。一种侧向流动免疫分析（LFIA）可能对广州管圆线虫病的防控有用。我们基于两种针对广州管圆线虫成虫抗原的单克隆抗体（mAbs）建立了一种用于诊断广州管圆线虫病的LFIA。LFIA的敏感性和特异性分别为91.1%和100%，而商用ELISA试剂盒的敏感性和特异性分别为97.8%和86.3%。LFIA的约登指数为0.91，商用ELISA试剂盒的约登指数为0.84。LFIA对广州管圆线虫ES抗原的检测限为1 ng/ml。这种LFIA简单、快速、高度敏感且特异，为人类广州管圆线虫病的诊断开辟了一种替代方法。
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