Ontogenetic variation of volatiles and antioxidant activity in leaves of Astragalus compactus Lam. (Fabaceae).

The genus Astragalus is a rich source of a variety of biologically active compounds including phenols, saponins, polysaccharides and essential oils. The present study was conducted to determine ontogenetic variation of the volatile organic compounds as well as total phenolic contents and antioxidant activity in leaves of A. compactus. The leaves of plant were harvested at vegetative, flowering and fructification stages and were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent and the antioxidant capacity was evaluated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Different classes of volatile compounds were identified including alcohols, esters, hydrocarbons, sterols and terpenoides. Significant variation of these compounds was found during phenological stages of development. Sterols and hydrocarbons were the main components of essential oils at the vegetative stage. The presence of terpenoides (phytol) and alcohols (docosanol) was significant at the flowering stage. Fructification phase was characterized by the high content of sterols and hydrocarbons and absence of phytol. The antioxidant activity and phenolic content were related to the physiological stage and the highest amount detected at fructification phase. The ontogenetic variations of phenolic contents and antioxidant properties are largely contributed by climatic factors such as temperature and solar radiation.