Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Department of Plant Science, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Bioresour Technol. 2016 Oct;218:700-11. doi: 10.1016/j.biortech.2016.07.018. Epub 2016 Jul 7.
An electric field (EF) generator device was fabricated and applied to the treatment of Chlorella vulgaris ISC33 at three distinct concentrations before cultivation. The EF of moderate intensity (2.7kVcm(-1)) has a hormetic effect on algal growth. The highest growth stimulation of 51% was observed after 50min treatment of 0.4gL(-1) algal suspension. The influence of EF on the system was then studied from both theoretical and experimental perspectives. The growth rate increased with treatment time up to a maximum because of improved membrane permeability, and then declined afterwards due to peroxide accumulation in the medium. The contents of chlorophylls, carotenoids, soluble carbohydrates, lipids, and proteins were also measured to understand possible changes on algal metabolism. The EF treatment of algal suspension has no observable effect on the cell metabolism while both algal growth and metabolism was significantly affected by the inoculum size.
研制了一种电场（EF）发生器装置，并在培养前将其应用于三种不同浓度的普通小球藻 ISC33 的处理。中等强度的 EF（2.7kVcm（-1））对藻类生长具有兴奋效应。在处理 0.4gL（-1）藻悬浮液 50min 后，观察到最高生长刺激率为 51%。然后从理论和实验两个方面研究了 EF 对系统的影响。由于细胞膜通透性的提高，处理时间的增加导致生长速率增加，随后由于介质中过氧化物的积累而下降。还测量了叶绿素、类胡萝卜素、可溶性碳水化合物、脂质和蛋白质的含量，以了解藻类代谢可能发生的变化。藻悬浮液的 EF 处理对细胞代谢没有明显影响，而接种量对藻类生长和代谢有显著影响。
