Soleymani Robati Seyed Mojtaba, Nosrati Mohsen, Ghanati Faezeh, Hajnowrouzi Abazar, Grizeau Dominique, Dupré Catherine
Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Nantes University, CNRS, GEPEA, UMR 6144, F-44600, Saint-Nazaire, France.
Appl Microbiol Biotechnol. 2021 Apr;105(8):3339-3351. doi: 10.1007/s00253-021-11233-x. Epub 2021 Mar 30.
Finding an eco-friendly process for the decolorization of distillery wastewaters is a major concern. This study shows that the Chlorella vulgaris CCAP 211/19 strain can be used for color removal and direct production of oleaginous biomass. A response surface method was used for determining optimal operating conditions, including the dilution factor of industrial wastewater. The highest daily light supply values were the most efficient for color removal. The analysis of the microalgae physiological status confirmed that these colored waters could have a photoprotective action. Moreover, the increase in photosystem 2 activities of C. vulgaris CCAP 211/19 strain after short-term incubations in the presence of a synthetic melanoidin confirmed that this fraction is involved in the enhancement of lipid-enriched biomass production. The results show for the first time the stimulation effect of a melanoidin fraction on the lipid content and productivity by C. vulgaris. These results suggest that this approach may be used to design a closed loop, including water and CO recycling for the wastewater dilution and photosynthetic carbon fixation, respectively, while providing biomass for useful renewable algae-based feedstocks of potential interest for a distillery process. KEY POINTS: • Chlorella vulgaris cultures can be used for decolorization of distillery wastewaters. • Diluted distillery wastewaters stimulate biomass and lipid productivities. • Melanoidins, as well as distillery wastewater, stimulate photosynthetic activities.
寻找一种环保的酿酒厂废水脱色工艺是一个主要问题。本研究表明，普通小球藻CCAP 211/19菌株可用于脱色并直接生产含油生物质。采用响应面法确定最佳操作条件，包括工业废水的稀释倍数。每日最高光照供应量对脱色最为有效。对微藻生理状态的分析证实，这些有色水可能具有光保护作用。此外，在合成类黑素存在下短期培养后，普通小球藻CCAP 211/19菌株的光系统2活性增加，证实该部分参与了富含脂质的生物质产量的提高。结果首次表明了类黑素部分对普通小球藻脂质含量和生产力的刺激作用。这些结果表明，这种方法可用于设计一个闭环，分别包括用于废水稀释的水和CO循环以及光合碳固定，同时为酿酒厂工艺提供潜在感兴趣的有用可再生藻类基原料的生物质。要点：•普通小球藻培养物可用于酿酒厂废水的脱色。•稀释的酿酒厂废水刺激生物质和脂质生产力。•类黑素以及酿酒厂废水刺激光合活性。
