Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Agricultural Science and Engineering School, Liaocheng University, Liaocheng, 252059, China.
Sci Rep. 2023 Jul 11;13(1):11203. doi: 10.1038/s41598-023-38219-1.
Dezhou donkey is one of the excellent large donkey breeds in China. In our study, eight microsatellite markers were used to genotype from each of three populations of Chinese Dezhou donkeys: 67 individuals in Liaocheng (pop1), 103 individuals in Binzhou 1 (pop2), and 102 individuals in Binzhou 2 (pop3), in order to study the genetic diversity of these populations. A total of 213 alleles were detected, and the PIC results showed that eight loci were all highly polymorphic. The means of Ho and He of pop2 were the highest, which were 0.646 and 0.717, respectively. The PCoA analysis demonstrated that the samples from three conservation farms were mixed together. The phylogenetic tree showed that pop 2 and pop 3 were closely related. The phylogenetic tree results clustered that 272 donkeys were divided into six groups. AMOVA analysis showed that the genetic variation was mainly concentrated within the population and the genetic differentiation among the populations was low. Fst values between populations also indicated that genetic differentiation between populations was too small to be considered. This indicated a low probability of inbreeding within the population. And this also showed that the conservation and breeding of Dezhou donkeys in recent years have achieved excellent results. The investigation of genetic diversity in three Dezhou donkey original breeding farms can provide reference data for the selection and breeding of excellent breeds of Dezhou donkey.
德州驴是中国优秀的大型驴品种之一。在本研究中，我们使用了 8 个微卫星标记对中国德州驴的三个种群（聊城的 67 个个体、滨州 1 号的 103 个个体和滨州 2 号的 102 个个体）进行了基因分型，以研究这些种群的遗传多样性。共检测到 213 个等位基因，PIC 结果表明，8 个位点均高度多态。滨州 2 号群体的 Ho 和 He 均值最高，分别为 0.646 和 0.717。PCoA 分析表明，三个保种场的样本混合在一起。系统发育树显示，滨州 2 号和滨州 3 号种群关系密切。系统发育树结果将 272 头驴分为 6 组。AMOVA 分析表明，遗传变异主要集中在种群内，种群间的遗传分化较低。种群间的 Fst 值也表明，种群间的遗传分化太小，不能被认为是遗传分化。这表明群体内的近交可能性较低。这也表明，近年来德州驴的保护和繁殖取得了优异的成果。对三个德州驴原种场的遗传多样性调查可为德州驴优良品种的选育提供参考数据。
