Si Young-Jae, Choi Won Suk, Kim Young-Il, Lee In-Won, Kwon Hyeok-Il, Park Su-Jin, Kim Eun-Ha, Kim Se Mi, Kwon Jin-Jung, Song Min-Suk, Kim Chul-Joong, Choi Young-Ki
College of Medicine and Medical Research Institute, Chungbuk National University, 12 Gaeshin-Dong, Heungduk-Ku, Cheongju, 361-763, Republic of Korea.
College of Veterinary Medicine, Chungnam National University, 220 Gung-Dong, Yuseoung-Gu, Daejeon, 305-764, Republic of Korea.
Arch Virol. 2016 Oct;161(10):2749-64. doi: 10.1007/s00705-016-2979-4. Epub 2016 Jul 16.
The continuous worldwide spread of highly pathogenic avian influenza (HPAI) H5N8 viruses among wild birds and poultry is a potential threat to public health. In the present study, we investigated the genetic characteristics of recent H5N8 viruses continuously isolated from migratory birds over two winters (2013-2014 and 2014-2015) in South Korea. Genetic and phylogenetic analysis demonstrated that the 2014-2015 HPAI H5N8 viruses are closely related to the 2013-2014 viruses, including virulence markers; however, all eight gene segments of 2014-2015 H5N8 viruses clustered in different phylogenetic branches from 2013-2014 H5N8 viruses, except the A/Em/Korea/W492/2015 virus. The H5N8 viruses of Europe and North America belong to sublineages of the 2013-2014 Korean H5N8 viruses but differ from the 2014-2015 Korean H5N8 viruses. Further hemagglutination inhibition (HI) assay results showed that there were 2-to-4 fold differences in HI titer between 2013-2014 and 2014-2015 H5N8 viruses. Taken together, our results suggested that the 2014-2015 Korean H5N8 viruses were genetically and serologically different from those of 2013-2014 winter season H5N8 viruses, including those from Europe and North America.
高致病性禽流感（HPAI）H5N8病毒在全球范围内持续在野生鸟类和家禽中传播，对公众健康构成潜在威胁。在本研究中，我们调查了韩国在两个冬季（2013 - 2014年和2014 - 2015年）从候鸟中持续分离出的近期H5N8病毒的基因特征。遗传和系统发育分析表明，2014 - 2015年的高致病性禽流感H5N8病毒与2013 - 2014年的病毒密切相关，包括毒力标记；然而，2014 - 2015年H5N8病毒的所有八个基因片段，除了A/Em/Korea/W492/2015病毒外，都聚集在与2013 - 2014年H5N8病毒不同的系统发育分支中。欧洲和北美的H5N8病毒属于2013 - 2014年韩国H5N8病毒的亚系，但与2014 - 2015年韩国H5N8病毒不同。进一步的血凝抑制（HI）试验结果表明，2013 - 2014年和2014 - 2015年H5N8病毒之间的HI效价存在2至4倍的差异。综上所述，我们的结果表明，2014 - 2015年韩国H5N8病毒在基因和血清学上与2013 - 2014年冬季季节的H5N8病毒不同，包括来自欧洲和北美的病毒。
