National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Plant Cell Rep. 2016 Oct;35(10):2167-79. doi: 10.1007/s00299-016-2027-6. Epub 2016 Jul 18.
Dual function of GhATAF1 in the responses to salinity stress and Verticillium dahliae infection in cotton. NAC (NAM/ATAF1/2/CUC2) is a large plant-specific transcription factor family that plays important roles in the response to abiotic stresses. We previously isolated a cotton NAC transcription factor gene, GhATAF1, which was up-regulated by ABA, cold and salt stresses and classified into AFAT1/2, a sub-family of NAC. Here, we report that GhATAF1 was also highly induced by MeJA, SA and Verticillium dahliae inoculation, which implied that GhATAF1 was involved not only in the response to abiotic stress but also in the response to biotic stress. GhATAF1 was localized in the nucleus and possessed transactivation activity. Overexpression of GhATAF1 enhanced cotton plant tolerance to salt stress by enhancing the expression of various stress-related genes, including the ABA response gene GhABI4; the transporter gene GhHKT1, involved in Na(+)/K(+) homeostasis; and several stress-response genes (GhAVP1, GhRD22, GhDREB2A, GhLEA3, and GhLEA6). Additionally, overexpressing GhATAF1 increased cotton plant susceptibility to the fungal pathogens V. dahliae and Botrytis cinerea, coupled with the suppression of JA-mediated signaling and the activation of SA-mediated signaling. Our results suggested that GhATAF1, the cotton stress-responsive NAC transcription factor, plays important roles in the response to both abiotic stress and biotic stress by coordinating the phytohormone signaling networks.
GhATAF1 在棉花耐盐性和黄萎病菌侵染中的双重功能。NAC（NAM/ATAF1/2/CUC2）是一个大型的植物特异性转录因子家族，在非生物胁迫响应中发挥重要作用。我们之前分离了一个棉花 NAC 转录因子基因 GhATAF1，它受 ABA、冷和盐胁迫诱导上调，并被归类为 NAC 的 AFAT1/2 亚家族。在这里，我们报告 GhATAF1 也被 MeJA、SA 和黄萎病菌接种高度诱导，这表明 GhATAF1 不仅参与非生物胁迫响应，还参与生物胁迫响应。GhATAF1 定位于细胞核内，并具有转录激活活性。过表达 GhATAF1 通过增强各种与应激相关的基因的表达，包括 ABA 反应基因 GhABI4；参与 Na(+)/K(+) 稳态的转运基因 GhHKT1；和几个应激响应基因（GhAVP1、GhRD22、GhDREB2A、GhLEA3 和 GhLEA6），增强棉花植株对盐胁迫的耐受性。此外，过表达 GhATAF1 增加了棉花植株对真菌病原体黄萎病菌和灰葡萄孢的易感性，同时抑制了 JA 介导的信号转导和激活了 SA 介导的信号转导。我们的研究结果表明，棉花应激响应 NAC 转录因子 GhATAF1 通过协调植物激素信号网络，在应对非生物胁迫和生物胁迫方面发挥重要作用。
