Lee Ji Young, Kim Byung-Kwon, Kang Mijeong, Park Jun Hui
Korea Advanced Institute of Science and Technology, Department of Chemistry, Daejeon, 34141, South Korea.
Sookmyung Women's University, Department of Chemistry, Seoul, 04310, South Korea.
Sci Rep. 2016 Jul 20;6:30022. doi: 10.1038/srep30022.
We detected single living bacterial cells on ultramicroelectrode (UME) using a single-particle collision method and optical microscopic methods. The number of collision events involving the bacterial cells indicated in current-time (i-t) curves corresponds to the number of bacterial cells (i.e., Escherichia coli) on the UME surface, as observed visually. Simulations were performed to determine the theoretical current response (75 pA) and frequency (0.47 pM(-1) s(-1)) of single Escherichia coli collisions. The experimental current response (83 pA) and frequency (0.26 pM(-1) s(-1)) were on the same order of magnitude as the theoretical values. This single-particle collision approach facilitates detecting living bacteria and determining their concentration in solution and could be widely applied to studying other bacteria and biomolecules.
我们使用单粒子碰撞方法和光学显微镜方法在超微电极（UME）上检测单个活细菌细胞。如通过视觉观察到的，电流-时间（i-t）曲线中显示的涉及细菌细胞的碰撞事件数量与UME表面的细菌细胞（即大肠杆菌）数量相对应。进行了模拟以确定单个大肠杆菌碰撞的理论电流响应（75 pA）和频率（0.47 pM⁻¹ s⁻¹）。实验电流响应（83 pA）和频率（0.26 pM⁻¹ s⁻¹）与理论值处于同一数量级。这种单粒子碰撞方法有助于检测活细菌并确定其在溶液中的浓度，并且可广泛应用于研究其他细菌和生物分子。
