Tzounakas Vassilis L, Kriebardis Anastasios G, Georgatzakou Hara T, Foudoulaki-Paparizos Leontini E, Dzieciatkowska Monika, Wither Matthew J, Nemkov Travis, Hansen Kirk C, Papassideri Issidora S, D'Alessandro Angelo, Antonelou Marianna H
Department of Biology, Section of Cell Biology and Biophysics, School of Science, NKUA, Athens 15784, Greece.
Laboratory of Hematology and Transfusion Medicine, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens, Athens 12210, Greece.
Data Brief. 2016 Jun 23;8:618-27. doi: 10.1016/j.dib.2016.06.018. eCollection 2016 Sep.
This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage in standard blood bank conditions compared to control, G6PD sufficient (G6PD(+)) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in RBCs throughout the storage period, with or without stimulation by oxidants, supplementation of N-acetylcysteine and energy depletion, following incubation of stored cells for 24 h at 37 °C. Apart from cell characteristics, the total or uric acid-dependent antioxidant capacity of the supernatant in addition to extracellular potassium concentration was determined in RBC units. Finally, procoagulant activity and protein carbonylation levels were measured in the microparticles population. Further information can be found in "Glucose 6-phosphate dehydrogenase deficient subjects may be better "storers" than donors of red blood cells" [1].
本文包含了与对照的、葡萄糖-6-磷酸脱氢酶（G6PD）充足（G6PD(+)）的红细胞相比，符合条件的G6PD缺乏的献血者所捐献的红细胞在标准血库条件下储存期间几种生理参数变化的数据。在储存期间，无论有无氧化剂刺激、补充N-乙酰半胱氨酸和能量耗竭，在37℃孵育储存细胞24小时后，对红细胞进行细胞内活性氧（ROS）生成、细胞脆性和膜外囊泡形成的检测。除细胞特征外，还测定了红细胞单位上清液的总抗氧化能力或尿酸依赖性抗氧化能力以及细胞外钾浓度。最后，测定了微粒群体中的促凝活性和蛋白质羰基化水平。更多信息可在《葡萄糖-6-磷酸脱氢酶缺乏的受试者可能比红细胞献血者更适合“储存”》[1]中找到。
