Assessment of accuracy of intraesophageal pH probe in a dog model.

We performed an experiment in dogs in order to assess the accuracy of an intraesophageal pH probe and to examine possible mechanisms for any lack of accuracy. Two pH probes were placed in the esophagus of supine anesthetized dogs and acid was infused in small serial volumes from an infusion tube placed at the gastroesophageal junction. Two general patterns of pH probe responses were seen: (1) neither probe recorded a fall in pH with acid infusion, and (2) both probes recorded a fall in pH, but in a nonsynchronous manner. Changing the relationship of the tips of the probes with respect to the adjacent mucosa caused a fall in the pH recorded by each probe to the pH of the intraesophageal infusate. This and other evidence seen in the pH tracings suggest that mucosal abutment of the probe tips causes a falsely negative pH recording and raises the question of whether such a phenomenon occurs in human studies.