Mao Bingyong, Li Dongyao, Ai Chunqing, Zhao Jianxin, Zhang Hao, Chen Wei
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University , 116034 Dalian, People's Republic of China.
Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University , 102488 Beijing, People's Republic of China.
J Agric Food Chem. 2016 Aug 10;64(31):6240-7. doi: 10.1021/acs.jafc.6b02305. Epub 2016 Aug 2.
In this study, C57BL/6J mice were fed diets supplemented with different proportions of lactulose (0%, 5%, and 15%) for 2 weeks to study its effects on the luminal and mucosal microbiota. The luminal and mucosal samples of cecum and colon were investigated. After high-lactulose treatment (15%), pH of the luminal contents decreased from 6.90-7.72 to 5.95-6.21 from the cecum to distal colon, and the amount of total short-chain fatty acids in the cecum was significantly increased. The luminal content was mostly dominated by Firmicutes, Actinobacteria, and Bacteroidetes, while the mucus was dominated by Firmicutes, Proteobacteria, and Bacteroidetes. The abundance of Actinobacteria was significantly increased in the content, and Proteobacteria was the most abundant phylum (∼50%) in the mucus after high-lactulose treatment. At the genus level, Bifidobacterium and Akkermansia were both significantly increased in the content, and Helicobacter was the most abundant in the mucus.
在本研究中，给C57BL/6J小鼠喂食添加不同比例乳果糖（0%、5%和15%）的日粮2周，以研究其对肠腔和黏膜微生物群的影响。对盲肠和结肠的肠腔和黏膜样本进行了研究。高剂量乳果糖（15%）处理后，从盲肠到结肠远端，肠腔内容物的pH值从6.90 - 7.72降至5.95 - 6.21，盲肠中总短链脂肪酸的量显著增加。肠腔内容物主要由厚壁菌门、放线菌门和拟杆菌门主导，而黏液则由厚壁菌门、变形菌门和拟杆菌门主导。高剂量乳果糖处理后，放线菌门在内容物中的丰度显著增加，变形菌门是黏液中最丰富的菌门（约50%）。在属水平上，双歧杆菌属和阿克曼氏菌属在内容物中均显著增加，幽门螺杆菌在黏液中最为丰富。
