State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2019 Oct 12;11(10):2431. doi: 10.3390/nu11102431.
Fructooligosaccharides (FOS) are considered prebiotics and have been proven to selectively promote the growth of in the gut. This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice. Briefly, mice were fed a 0% or 25% FOS (/)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the was analyzed by sequencing the V3-V4 region of 16S rRNA and gene, respectively. After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice. At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group. Besides , a significant increase was observed in the relative abundance of in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents. Furthermore, sequencing revealed that was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%). These results indicated that FOS can selectively promote proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon. Further studies are required to reveal the competitive advantage of over other FOS-metabolizing bacteria and the response mechanisms of to FOS.
低聚果糖（FOS）被认为是一种益生元，已被证明能选择性地促进肠道内的生长。本研究旨在阐明 FOS 摄入对小鼠肠道腔和黏膜微生物群落组成的影响。简而言之，将小鼠喂食 0%或 25% FOS（-）补充饮食 4 周，分别通过测序 16S rRNA 和基因的 V3-V4 区来分析腔和黏膜微生物群落的组成，特别是。在 FOS 干预后，小鼠盲肠的总重量和壁重量以及盲肠内容物中总短链脂肪酸（SCFA）的量均显著增加。在门水平上，结果表明 FOS 组内容物和从盲肠到远端结肠的黏膜中放线菌的相对丰度显著增加。此外，在属水平上，所有样本中相对丰度均显著增加，这可能与腔内容物中丁酸水平的增加部分相关。此外，测序结果表明，在腔内容物（>98%）和黏膜（>89%）中，几乎是唯一的双歧杆菌属物种。这些结果表明，FOS 可以选择性地促进肠道内的增殖，无论是在腔中还是从盲肠到远端结肠的黏膜中。需要进一步的研究来揭示对其他 FOS 代谢细菌的竞争优势和对 FOS 的反应机制。
