Wang Jing, Liu Xiangming, Jiang Zhongmin, Li Lili, Cui Zhigang, Gao Yuan, Kong Di, Liu Xiaozhi
Department of Tumor Surgery, Tianjin Nankai Hospital, Tianjin 300100, P.R. China.
Department of Esophageal Neoplasms, Cancer Hospital of Tianjin Medical University, Tianjin 300060, P.R. China.
Oncol Lett. 2016 Aug;12(2):1355-1360. doi: 10.3892/ol.2016.4757. Epub 2016 Jun 22.
The majority of cancer stem cells exist in the G0, or quiescent phase of the cell cycle. However, the cells can escape quiescence following routine radiotherapy and chemotherapy, resulting in tumor recurrence. Presently, achieving the accurate regulation of cancer stem cell growth in order to study a specific state, including the quiescent (mostly G0 or G1 phase), proliferative (mostly S phase) or differential (mostly G2/M phase) states, can be challenging. This makes the determination of cell cycle state-specific characteristics and analysis of potential intervention treatments difficult, particularly for quiescent cells. Breast cancer stem cells were cultured on a soft or hard agar matrix surface in the presence or absence of stem cell growth factors. Cells could be successfully limited in either the quiescent, proliferative or differentiated states. These findings provide a foundation for further study of the cell cycle in breast cancer stem cells.
大多数癌症干细胞处于细胞周期的G0期或静止期。然而，这些细胞在常规放疗和化疗后可逃离静止状态，导致肿瘤复发。目前，要实现对癌症干细胞生长的精确调控以研究特定状态，包括静止（主要是G0或G1期）、增殖（主要是S期）或分化（主要是G2/M期）状态，可能具有挑战性。这使得确定细胞周期状态特异性特征以及分析潜在干预治疗变得困难，尤其是对于静止细胞。乳腺癌干细胞在有无干细胞生长因子的情况下，培养于软或硬琼脂基质表面。细胞能够成功地被限制在静止、增殖或分化状态。这些发现为进一步研究乳腺癌干细胞的细胞周期奠定了基础。
