Liu Wei-Jie, Peng Yu-Yuan, Chen Hao, Liu Xian-Fang, Liang Jing-Yu, Sun Jian-Bo
Department of Natural Medicinal Chemistry, China Pharmaceutical University, #24 Tongjiaxiang, Nanjing, 210009, P. R. China.
Chem Biodivers. 2017 Feb;14(2). doi: 10.1002/cbdv.201600209. Epub 2017 Jan 10.
A new 19-oxo-18,19-seco-ursane-type triterpeonoid saponin, laevigin E (8), together with 17 known compounds (1 - 7 and 9 - 18) were isolated from the root bark of Ilex rotunda Thunb. Their structures were determined by various spectroscopic analysis. Among them, compounds 6, 9, 11, and 18 were isolated from this species for the first time, while compounds 10 and 12 were firstly isolated from the family Aquifoliaceae. Biological activity assay showed that all triterpenoids exhibit moderate cytotoxic activities against MCF7, A549, HeLa and LN229 cell lines. The four triterpenoid saponins (3, 4, 6, and 8) exhibit slightly better activities compared to the four triterpenoid sapogenins (1, 2, 5, and 7). Compound 8 showed the best cytotoxicity against A549, HeLa and LN229 cell lines with IC of 17.83, 22.58 and 30.98 μm, respectively.
从铁冬青的根皮中分离得到一种新的19-氧代-18,19-断乌苏烷型三萜皂苷——光冬青苷E（8），以及17个已知化合物（1 - 7和9 - 18）。通过各种光谱分析确定了它们的结构。其中，化合物6、9、11和18首次从该物种中分离得到，而化合物10和12首次从冬青科植物中分离得到。生物活性测定表明，所有三萜类化合物对MCF7、A549、HeLa和LN229细胞系均表现出中等程度的细胞毒性活性。与四种三萜皂苷元（1、2、5和7）相比，四种三萜皂苷（3、4、6和8）表现出稍好的活性。化合物8对A549、HeLa和LN229细胞系表现出最佳的细胞毒性，其IC50分别为17.83、22.58和30.98μm。
