通过聚合酶链反应将人类补体成分C9的基因定位到5号染色体上。

The gene for human complement component C9 mapped to chromosome 5 by polymerase chain reaction.

作者信息

Abbott C, West L, Povey S, Jeremiah S, Murad Z, DiScipio R, Fey G

机构信息

MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, United Kingdom.

出版信息

Genomics. 1989 May;4(4):606-9. doi: 10.1016/0888-7543(89)90286-3.

DOI:10.1016/0888-7543(89)90286-3

PMID:2744767

Abstract

The gene for human complement component C9 has been mapped to chromosome 5. This was achieved by using a novel application of the polymerase chain reaction to amplify specifically the human C9 gene on a background of rodent DNA in somatic cell hybrids. The assignment to chromosome 5 was confirmed by in situ hybridization to human metaphase chromosomes, giving a regional localization of 5p13.

摘要

人类补体成分C9的基因已被定位到5号染色体。这是通过一种聚合酶链反应的新应用来实现的，即在体细胞杂种中的啮齿动物DNA背景下特异性扩增人类C9基因。通过与人类中期染色体的原位杂交证实了该基因定位于5号染色体，其区域定位为5p13。