Setién F, Alvarez V, Coto E, DiScipio R G, López-Larrea C
Servicio de Inmunologia, Hospital Central Universitario de Asturias, Spain.
Immunogenetics. 1993;38(5):341-4. doi: 10.1007/BF00210475.
The genes for human complement components C6, C7, and C9 are linked on chromosome 5. In this report we describe the physical linkage between C6 and C7 genes. DNA fragments obtained by digestion with several rare-cutting restriction enzymes were separated through pulsed field gel electrophoresis. Hybridization with probes corresponding to the 5' and 3' ends of the three cDNAs showed common bands for the C6 and C7 genes. Both genes are contained in a NotI fragment of 500 kilobases (kb). Moreover, the presence of common 3' C6 and 3' C7 fragments indicates that both genes are oriented in a tail-to-tail, reverse way relative to transcription. No evidence of physical linkage between C9 and C6 or C7 was found in the range 50 kb-2.5 megabases (Mb).
人类补体成分C6、C7和C9的基因在5号染色体上连锁。在本报告中，我们描述了C6和C7基因之间的物理连锁关系。用几种稀有切割限制酶消化获得的DNA片段通过脉冲场凝胶电泳进行分离。与对应于三种cDNA 5'和3'末端的探针杂交显示C6和C7基因有共同条带。两个基因都包含在一个500千碱基（kb）的NotI片段中。此外，共同的3' C6和3' C7片段的存在表明两个基因相对于转录以尾对尾的反向方式排列。在50 kb至2.5兆碱基（Mb）范围内未发现C9与C6或C7之间存在物理连锁的证据。
