Ben Salem Issam, Mezni Mohamed, Boulila Abdennacer, Hamdi Mokhtar, Saidi Mouldi
Laboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Science and Technology (CNSTN), Sidi Thabet Technopark, 2020, Ariana, Tunisia.
Methods and Analysis Techniques Laboratory LR 10 INRAP 03, National Institute of Research and Physicochemical Analysis, 2020, Sidi Thabet, Tunisia.
Curr Microbiol. 2016 Oct;73(4):582-6. doi: 10.1007/s00284-016-1097-6. Epub 2016 Jul 22.
The decomposition of penicillin G and erythromycin antibiotics at concentration of 0.2 mg ml(-1) by gamma irradiation at 50 kGy followed by biological treatment with Cupriavidus metallidurans CH34 was evaluated. Degradation of penicillin G and erythromycin was analyzed using nuclear magnetic resonance analysis (NMR), fourier transform infrared spectroscopy (FTIR), and chemical oxygen demand (COD). The exposure to the absorbed dose of 50 kGy caused degradation of penicillin G and erythromycin in the aqueous solution. The complete disappearance of NMR and FTIR peaks following irradiation confirmed the breakage of the β-lactam ring in penicillin G, and the decarboxylation and cleavage of the thiazolidine ring and for erythromycin, the complete destruction of the three aromatic rings. Irradiation alone removed 52.8 and 65.5 % of penicillin G and erythromycin, respectively. Further reduction to 12.6 and 14 % of the original penicillin G and erythromycin COD, respectively, was achieved using treatment of the irradiation products with C. metallidurans.
评估了在50 kGy的γ辐照下浓度为0.2 mg ml⁻¹的青霉素G和红霉素抗生素的分解情况，随后用金属抗性贪铜菌CH34进行生物处理。使用核磁共振分析（NMR）、傅里叶变换红外光谱（FTIR）和化学需氧量（COD）分析青霉素G和红霉素的降解情况。暴露于50 kGy的吸收剂量会导致水溶液中青霉素G和红霉素的降解。辐照后NMR和FTIR峰的完全消失证实了青霉素G中β-内酰胺环的断裂，以及红霉素中噻唑烷环的脱羧和裂解，以及三个芳香环的完全破坏。单独辐照分别去除了52.8%和65.5%的青霉素G和红霉素。使用金属抗性贪铜菌处理辐照产物后，青霉素G和红霉素的COD分别进一步降低至原来的12.6%和14%。
