Aquino R, Morelli S, Lauro M R, Abdo S, Saija A, Tomaino A
Dipartimento di Scienze Farmaceutiche and Scuola di Specializzazione in Scienza e Tecnologia Cosmetiche, Università di Salerno, Invariante 11/C, 84084 Fisciano, Salerno, Italy.
J Nat Prod. 2001 Aug;64(8):1019-23. doi: 10.1021/np0101245.
Fractionation of a methanolic extract of the leaves of Anthurium versicolor has resulted in the isolation of two main fractions, I and II. Both the extract and the fractions were assayed for their radical-scavenging activity by means of an in vitro test (bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical) and showed a significant radical-scavenging effect. Subsequent chromatographic fractionation of the most active fraction, II, has led to the isolation and characterization, as major constituents, of four new flavone glycosides, acacetin 6-C-[alpha-L-rhamnopyranosyl-(1-->3)-beta-D-glucopyranoside] (1), acacetin 6-C-[beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranoside] (2), acacetin 6-C-[beta-D-apiofuranosyl-(1-->3)-beta-D-glucopyranoside] (3), and acacetin 8-C-[alpha-L-rhamnopyranosyl-(1-->3)-beta-D-glucopyranoside] (4), as well as vitexin (apigenin-8-C-beta-D-glucopyranoside) and rosmarinic acid. The structures of 1-4 were determined using spectroscopic methods.
对变色花烛叶片的甲醇提取物进行分馏，得到了两个主要馏分，即馏分I和馏分II。通过体外试验（稳定的1,1-二苯基-2-苦基肼自由基的漂白）对提取物和馏分的自由基清除活性进行了测定，结果显示它们具有显著的自由基清除效果。随后，对活性最高的馏分II进行色谱分馏，分离并鉴定出四种新的黄酮苷作为主要成分，分别是刺槐素6-C-[α-L-鼠李糖基-(1→3)-β-D-葡萄糖苷]（1）、刺槐素6-C-[β-D-木糖基-(1→6)-β-D-葡萄糖苷]（2）、刺槐素6-C-[β-D-芹菜糖基-(1→3)-β-D-葡萄糖苷]（3）和刺槐素8-C-[α-L-鼠李糖基-(1→3)-β-D-葡萄糖苷]（4），以及牡荆素（芹菜素-8-C-β-D-葡萄糖苷）和迷迭香酸。通过光谱方法确定了1-4的结构。
