Smith L, Andreasson S, Bergdahl S, Rippe B, Risberg B
Department of Surgery I, University of Göteborg, Sweden.
J Appl Physiol (1985). 1989 Jun;66(6):2866-72. doi: 10.1152/jappl.1989.66.6.2866.
Changes in pulmonary microvascular permeability in sheep, after oleic acid injection, were studied using estimations of the osmotic reflection coefficient (sigma d) for total protein, albumin, immunoglobulins (Ig) G and M and calculation of the equivalent small and large pores of the microvessels. A chronic lung fistula was prepared in eight sheep. After a base-line period, left atrial pressure (Pla) was increased. Oleic acid (0.05 mg/kg body wt) was injected after a filtration-independent state had been obtained, and the spontaneously ventilating animals were then followed for 2 h. The sigma d for the normal lung was 0.65 +/- 0.03, 0.59 +/- 0.02, 0.72 +/- 0.04, and 0.84 +/- 0.02 for total protein, albumin, IgG, and IgM, respectively. The equivalent pore radii were 54 and 225 A. After oleic acid infusion, arterial pressure and arterial O2 tension decreased and leukocytes and platelets were consumed. At the end of the experiment, sigma d's were 0.27 +/- 0.04, 0.24 +/- 0.07, 0.33 +/- 0.06, and 0.55 +/- 0.04 for total protein, albumin, IgG, and IgM, respectively. The equivalent pore radii were 54 and 275 A, and the number of large pores was increased by 195%. The results indicate that oleic acid produces an increased vascular permeability by increasing the size and the numbers of large pores of the pulmonary microvascular walls.
通过测定总蛋白、白蛋白、免疫球蛋白（Ig）G和M的渗透反射系数（σd）以及计算微血管等效小孔和大孔，研究了油酸注射后绵羊肺微血管通透性的变化。在八只绵羊身上制备了慢性肺瘘。在基线期后，升高左心房压力（Pla）。在获得与滤过无关的状态后注射油酸（0.05mg/kg体重），然后对自主呼吸的动物观察2小时。正常肺组织中总蛋白、白蛋白、IgG和IgM的σd分别为0.65±0.03、0.59±0.02、0.72±0.04和0.84±0.02。等效孔半径分别为54和225埃。注入油酸后，动脉压和动脉血氧张力降低，白细胞和血小板消耗。实验结束时，总蛋白、白蛋白、IgG和IgM的σd分别为0.27±0.04、0.24±0.07、0.33±0.06和0.55±0.04。等效孔半径分别为54和275埃，大孔数量增加了195%。结果表明，油酸通过增加肺微血管壁大孔的尺寸和数量，导致血管通透性增加。
