Smith L, Andreasson S, Thorén-Tolling K, Rippe B, Risberg B
J Appl Physiol (1985). 1987 Apr;62(4):1422-9. doi: 10.1152/jappl.1987.62.4.1422.
The changes in pulmonary microvascular permeability in sheep, after infusion of live Escherichia coli, were studied using estimations of the osmotic reflection coefficients (sigma) for total protein, albumin, immunoglobins (Ig) G and M and based on these estimations equivalent pore dimensions were calculated. A chronic lung lymph fistula was prepared in seven sheep. After a base-line period, left atrial pressure (Pla) was increased. E. coli (10(9) X kg body wt) were given after attaining filtration independent L/P values. The sigma's for the normal lung were calculated to 0.73 for total protein and to 0.65, 0.76, and 0.91 for albumin, IgG, and IgM, respectively. The equivalent pore radii were determined to 50 and 175 A with 35% of the filtration accounted for by the large pores. After bacterial infusion, the sigma's for total protein, albumin, IgG, and IgM decreased significantly from preseptic values to 0.58, 0.50, 0.64, and 0.83, respectively. After sepsis the small pores were 50 A and the large pores 200 A with 49% of total volume flow at maximum lymph flows occurring through the large pores. Assuming a constant small-pore population the large-pore number increased 32% after bacterial infusion. These results indicate that pulmonary microvascular permeability may have increased due to the sepsis.
通过估计总蛋白、白蛋白、免疫球蛋白G和M的渗透反射系数（σ），并基于这些估计值计算等效孔径尺寸，研究了绵羊输注活大肠杆菌后肺微血管通透性的变化。对7只绵羊制备了慢性肺淋巴瘘。在基线期后，升高左心房压力（Pla）。在达到滤过无关的L/P值后给予大肠杆菌（10⁹×kg体重）。计算出正常肺的总蛋白σ为0.73，白蛋白、IgG和IgM的σ分别为0.65、0.76和0.91。等效孔径半径确定为50和175埃，其中35%的滤过由大孔负责。细菌输注后，总蛋白、白蛋白、IgG和IgM的σ从败血症前的值显著降低至分别为0.58、0.50、0.64和0.83。败血症后，小孔为50埃，大孔为200埃，在最大淋巴流量时总流量的49%通过大孔。假设小孔数量恒定，细菌输注后大孔数量增加32%。这些结果表明，败血症可能导致肺微血管通透性增加。
