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急性弓形虫病期间尿液中抗原的检测。

Detection of antigens in urine during acute toxoplasmosis.

作者信息

Huskinson J, Stepick-Biek P, Remington J S

机构信息

Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.

出版信息

J Clin Microbiol. 1989 May;27(5):1099-101. doi: 10.1128/jcm.27.5.1099-1101.1989.

DOI:10.1128/jcm.27.5.1099-1101.1989
PMID:2745683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267492/
Abstract

Toxoplasma antigens were detected in sera and urine of mice acutely infected with Toxoplasma gondii. The concentrations of antigens in the urine samples measured by enzyme-linked immunosorbent assay were similar to those detected in the sera of the corresponding mice. The major antigens were not dialyzable and were largely destroyed by treatment with trichloroacetic acid and heat (100 degrees C for 1 h). Toxoplasma antigens were demonstrable on Western blots (immunoblots) of the urine samples.

摘要

在急性感染刚地弓形虫的小鼠血清和尿液中检测到弓形虫抗原。通过酶联免疫吸附测定法测得的尿液样本中抗原浓度与相应小鼠血清中检测到的浓度相似。主要抗原不可透析，且经三氯乙酸处理和加热（100℃ 1小时）后大部分被破坏。在尿液样本的蛋白质印迹法（免疫印迹）中可证实存在弓形虫抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddee/267492/6921cf64ab1b/jcm00065-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddee/267492/6921cf64ab1b/jcm00065-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddee/267492/6921cf64ab1b/jcm00065-0324-a.jpg

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本文引用的文献

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Use of monoclonal antibodies to detect antigens of Toxoplasma gondii in serum and other body fluids.使用单克隆抗体检测血清和其他体液中弓形虫的抗原。
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Antigen(s) responsible for immunoglobulin G responses specific for the acute stage of Toxoplasma infection in humans.引发人体针对弓形虫感染急性期特异性免疫球蛋白G反应的抗原。
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