Suzuki Y, Thulliez P, Desmonts G, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
J Clin Microbiol. 1988 May;26(5):901-5. doi: 10.1128/jcm.26.5.901-905.1988.
An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglutination test), positive results were obtained during both the acute and chronic (latent) stages of the infection. Studies were performed to define the antigen(s) of T. gondii which are detected by IgG antibodies present only during the acute stage of the infection. Sera of mice immunized with AC antigens recognized predominantly 10 antigens of tachyzoites by immunoblot analysis. Sera from individuals with the acute but not chronic infection reacted strongly with these same 10 antigens in immunoblots. Sera of mice immunized with HS antigens recognized more Toxoplasma antigens on immunoblots than did mouse AC antibodies. Absorption of the latter with HS antigens removed detectable reactivity of AC antibodies in immunoblots and in the AC and HS agglutination tests, suggesting that AC antigens are a selected portion of HS antigens. AC antigens were specific for tachyzoites in that AC antibodies reacted with the cell membranes of both forms of the organism in an indirect fluorescent-antibody test. Tachyzoite-specific antigen(s) appear to be useful in differentiating between the acute and chronic stages of Toxoplasma infection through their detection by IgG antibodies.
用丙酮固定速殖子（AC抗原；AC凝集试验）进行免疫球蛋白G（IgG）弓形虫抗体凝集试验时，仅在感染急性期患者的血清中呈阳性。相比之下，用福尔马林固定速殖子（HS抗原；HS凝集试验）进行该试验时，在感染的急性期和慢性（潜伏）期均获得阳性结果。开展研究以确定仅在感染急性期出现的IgG抗体所检测到的弓形虫抗原。通过免疫印迹分析，用AC抗原免疫的小鼠血清主要识别速殖子的10种抗原。急性感染而非慢性感染个体的血清在免疫印迹中与这相同的10种抗原发生强烈反应。用HS抗原免疫的小鼠血清在免疫印迹中识别的弓形虫抗原比小鼠AC抗体更多。用HS抗原吸收后者可消除免疫印迹以及AC和HS凝集试验中AC抗体的可检测反应性，这表明AC抗原是HS抗原的一个选定部分。AC抗原对速殖子具有特异性，因为在间接荧光抗体试验中，AC抗体与该生物体两种形态的细胞膜均发生反应。速殖子特异性抗原似乎可通过IgG抗体检测用于区分弓形虫感染的急性期和慢性期。
