Wu Bing-Li, Wang Dong, Bai Wen-Jing, Zhang Fan, Zhao Xing, Yi Ying, Zhang Ting, Shen Wen-Jun, Li En-Min, Xu Li-Yan, Xu Jian-Zhen
The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou 515041, China.
Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, China.
Oncotarget. 2016 Aug 30;7(35):56758-56766. doi: 10.18632/oncotarget.10779.
Cell death is a critical biological process involved in many important functions, and defects in this system are usually linked with numerous human diseases including cancers. Esophageal squamous cell carcinoma is one of the most chemo- and biological therapy resistant cancers. Based on knowledge repository and four miRNAs profiling data, we proposed a general framework to hunt for cell death miRNAs in a context dependent manner. We predicted 12 candidate miRNAs from hundreds of others. Follow-up experimental verification of 7 miRNAs indicated at least 3 miRNAs (MIR20b, MIR498 and MIR196) were involved in both apoptosis and autophagy processes. These results indicated miRNAs intimately connected the two cell death modules in esophageal squamous cell carcinoma. This integrative framework can also be easily extended to identify miRNAs in other key cellular signaling pathways or may find conditional specific miRNAs in other cancer types.
细胞死亡是一个涉及许多重要功能的关键生物学过程，该系统的缺陷通常与包括癌症在内的众多人类疾病相关。食管鳞状细胞癌是对化学疗法和生物疗法最具抗性的癌症之一。基于知识库和四个微小RNA（miRNA）谱数据，我们提出了一个通用框架，以依赖上下文的方式寻找细胞死亡相关的miRNA。我们从数百个其他miRNA中预测出12个候选miRNA。对7个miRNA的后续实验验证表明，至少有3个miRNA（MIR20b、MIR498和MIR196）参与了凋亡和自噬过程。这些结果表明，miRNA在食管鳞状细胞癌中紧密连接了两个细胞死亡模块。这个综合框架也可以很容易地扩展，以识别其他关键细胞信号通路中的miRNA，或者在其他癌症类型中找到条件特异性的miRNA。
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