Kim Soo-Jin, Cho Hayoung, Ahn Jae-Hyung, Weon Hang-Yeon, Seok Soon-Ja, Kim Jeong-Seon, Kwon Soon-Wo
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4167-4171. doi: 10.1099/ijsem.0.001330. Epub 2016 Jul 27.
A bacterial strain, designated T16R-265T, was isolated from rhizosphere of tomato in Buyeo-gun, Chungcheongnam-do, South Korea. The strain was Gram-stain-negative, aerobic, non-flagellated and rod- or long-rod-shaped. It grew at the range of 10-40 °C (optimum 28-30 °C), pH 6.0-9.0 (optimum pH 7.0) and with 0-3 % (w/v) NaCl (optimum 0 %). 16S rRNA gene sequence analysis showed that strain T16R-265T had the highest sequence similarity (98.0 %) with Flavitalea soli KIS20-3T and low sequence similarities of below 95 % with all other taxa. The phylogenetic tree revealed that the strain formed a robust monophyletic line with Flavitalea soli KIS20-3T separable from other clades. The predominant quinone of strain T16R-265T was MK-7, the polar lipids consisted of large amounts of phosphatidylethanolamine and an unknown lipid, and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. DNA-DNA hybridization values with Flavitalea soli KACC 17319T were below 70 %. The DNA G+C content of strain T16R-265T is 46.2 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain T16R-265T represents a novel species of a new genus, for which the name Pseudoflavitalea rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of the type species is T16R-265T (=KACC 18655T=NBRC 111880T). We also propose the reclassification of Flavitalea soli as Pseudoflavitalea soli comb. nov. (type strain KIS20-3T=KACC 17319T=JCM 19937T).
从韩国忠清南道扶余郡番茄根际分离出一株细菌，命名为T16R - 265T。该菌株革兰氏染色阴性，好氧，无鞭毛，呈杆状或长杆状。其生长温度范围为10 - 40°C（最适温度28 - 30°C），pH值为6.0 - 9.0（最适pH值7.0），NaCl浓度为0 - 3%（w/v）（最适浓度0%）。16S rRNA基因序列分析表明，菌株T16R - 265T与土壤黄杆菌KIS20 - 3T的序列相似性最高（98.0%），与所有其他分类单元的序列相似性均低于95%。系统发育树显示，该菌株与土壤黄杆菌KIS20 - 3T形成一个稳健的单系分支，与其他分支可区分开来。菌株T16R - 265T的主要醌类为MK - 7，极性脂质由大量磷脂酰乙醇胺和一种未知脂质组成，主要脂肪酸为异C15 : 0、异C17 : 0 3 - OH和异C15 : 1 G。与土壤黄杆菌KACC 17319T的DNA - DNA杂交值低于70%。菌株T16R - 265T的DNA G + C含量为46.2 mol%。基于系统发育证据以及表型、基因型和化学分类分析结果，菌株T16R - 265T代表一个新属的新物种，为此提出新属名假黄杆菌属（Pseudoflavitalea）和新种名根际假黄杆菌（Pseudoflavitalea rhizosphaerae）。模式种的模式菌株为T16R - 265T（=KACC 18655T=NBRC 111880T）。我们还建议将土壤黄杆菌（Flavitalea soli）重新分类为土壤假黄杆菌（Pseudoflavitalea soli）组合新名（模式菌株KIS20 - 3T=KACC 17319T=JCM 19937T）。
