Kim Soo-Jin, Cho Hayoung, Ahn Jae-Hyung, Weon Hang-Yeon, Joa Jae-Ho, Hong Seung-Beom, Seok Soon-Ja, Kim Jeong-Seon, Kwon Soon-Wo
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeju 63240, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3105-3110. doi: 10.1099/ijsem.0.002104.
A yellow, aerobic, Gram-stain-negative, non-motile, rod-shaped and non-flagellated bacterial strain, designated T16R-129T, was isolated from the rhizosphere of a tomato plant collected at a farm located on Buyeo-gun of Chungcheongnam-do, South Korea. Strain T16R-129T grew at 15-40 °C and pH 7.0-9.0, and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T16R-129T clustered with members of the genus Terrimonas, and it shared highest similarity with Terrimonas arctica R9-86T (96.1 %), Terrimonas pekingensis QHT (95.9 %), Terrimonas lutea DYT (94.9 %), Terrimonas crocea M1-33108T (95.4 %) and Terrimonas rhizosphaerae CR94T (95.3 %). The major isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. The polar lipids of strain T16R-129T were phosphatidylethanolamine, two unidentified aminolipids, two unidentified aminophospholipids and five unidentified polar lipids. The G+C content of the genomic DNA was 46.0 mol%. On the basis of data from this polyphasic taxonomic study, strain T16R-129T represents a novel species in the genus Terrimonas, for which the name Terrimonas terrae sp. nov. is proposed; the type strain is T16R-129T (=KACC 18787T=JCM 31603T).
从韩国忠清南道扶余郡一个农场采集的番茄植株根际中分离出一株黄色、需氧、革兰氏染色阴性、无运动性、杆状且无鞭毛的细菌菌株，命名为T16R - 129T。菌株T16R - 129T在15 - 40 °C和pH 7.0 - 9.0条件下生长，生长不需要NaCl。基于16S rRNA基因序列的系统发育分析表明，菌株T16R - 129T与地单胞菌属的成员聚类，与北极地单胞菌R9 - 86T（96.1 %）、北京地单胞菌QHT（95.9 %）、黄色地单胞菌DYT（94.9 %）、橙色地单胞菌M1 - 33108T（95.4 %）和根际地单胞菌CR94T（95.3 %）的相似性最高。主要类异戊二烯醌为甲基萘醌7（MK - 7）。主要细胞脂肪酸为异C15 : 0、异C17 : 0 3 - OH和异C15 : 1 G。菌株T16R - 129T的极性脂质为磷脂酰乙醇胺、两种未鉴定的氨基脂质、两种未鉴定的氨基磷脂和五种未鉴定的极性脂质。基因组DNA的G + C含量为46.0 mol%。基于这项多相分类研究的数据，菌株T16R - 129T代表地单胞菌属中的一个新物种，为此提议命名为土壤地单胞菌（Terrimonas terrae sp. nov.）；模式菌株为T16R - 129T（=KACC 18787T=JCM 31603T）。
