血红蛋白苏黎世[His E7(63)β被Arg取代]和悉尼[Val E11(67)β被Ala取代]的结构以及远端残基在配体结合中的作用。
Structure of hemoglobins Zürich [His E7(63)beta replaced by Arg] and Sydney [Val E11(67)beta replaced by Ala] and role of the distal residues in ligand binding.
作者信息
Tucker P W, Phillips S E, Perutz M F, Houtchens R, Caughey W S
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1076-80. doi: 10.1073/pnas.75.3.1076.
Abstract
In hemoglobin Zürich the side chain of the distal arginine attaches itself to the propionate of the heme, leaving the heme pocket wide open, allowing sulfanilamides easy access to the iron, and doubling the partition coefficient between CO and O2. The replacement of the distal valine by alanine in hemoglobin Sydney leaves a large gap inside the heme pocket, which is partly filled by a water molecule bonded to the distal histidine. Hemoglobin Sydney has the same partition coefficient between CO and O2 as hemoglobin A. Replacement of the distal histidine increases the stretching frequency of CO linked to the beta heme by 6 cm-1, but replacement of the distal valine increases it by only 3 cm-1, but replacement of the distal histidine leaves the O-O stretching frequency unchanged.
摘要
在苏黎世血红蛋白中，远端精氨酸的侧链与血红素的丙酸酯相连，使血红素口袋大开，让磺胺类药物易于接近铁，并使一氧化碳和氧气之间的分配系数加倍。在悉尼血红蛋白中，远端缬氨酸被丙氨酸取代，在血红素口袋内留下一个大空隙，部分被与远端组氨酸结合的水分子填充。悉尼血红蛋白在一氧化碳和氧气之间的分配系数与血红蛋白A相同。远端组氨酸的取代使与β-血红素相连的一氧化碳的伸缩频率增加6厘米-1，但远端缬氨酸的取代仅使其增加3厘米-1，而远端组氨酸的取代使氧-氧伸缩频率不变。
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本文引用的文献
1
X-ray and solubility studies of the haemoglobin of sickle-cell anaemia patients.镰状细胞贫血患者血红蛋白的X射线和溶解性研究。
Nature. 1951 Jun 9;167(4258):929-31. doi: 10.1038/167929a0.
2
3
Haemoglobin Zurich: alpha 2A beta 2-63 Arg.
Biochim Biophys Acta. 1961 Jul 8;50:595. doi: 10.1016/0006-3002(61)90028-2.
4
[Hemoglobin Zuerich: a new hemoglobin anomaly with sulfonamide-induced inclusion body anemia].
Helv Paediatr Acta. 1960 Dec;15:499-514.
5
Haemoglobin sydney: Beta-67 (E11) valine modified to alanine: an emerging pattern of unstable haemoglobins.
Nature. 1967 Aug 5;215(5101):626-8. doi: 10.1038/215626a0.
6
The matching of physical models to three-dimensional electron-density maps: a simple optical device.物理模型与三维电子密度图的匹配：一种简单的光学装置。
J Mol Biol. 1968 Oct 14;37(1):225-30. doi: 10.1016/0022-2836(68)90085-5.
7
Molecular pathology of human haemoglobin.人类血红蛋白的分子病理学
Nature. 1968 Aug 31;219(5157):902-9. doi: 10.1038/219902a0.
8
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
9
Nuclear magnetic resonance studies of hemoglobiss. V. The heme proton spectra of human deoxyhemoglobins A, F, Zurich, and Chesapeake.血红蛋白的核磁共振研究。V. 人类脱氧血红蛋白A、F、苏黎世型和切萨皮克型的血红素质子光谱。
Biochem Biophys Res Commun. 1970 Jul 27;40(2):343-9. doi: 10.1016/0006-291x(70)91015-6.
10
Three dimensional fourier synthesis of horse deoxyhaemoglobin at 2.8 Angstrom units resolution.分辨率为2.8埃单位的马脱氧血红蛋白的三维傅里叶合成。
Nature. 1970 Nov 7;228(5271):551-2. doi: 10.1038/228551a0.
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