珠蛋白信使核糖核酸序列在其16S前体中的位置。
The location of the globin mRNA sequence within its 16S precursor.
作者信息
Smith K, Rosteck P, Lingrel J B
出版信息
Nucleic Acids Res. 1978 Jan;5(1):105-15. doi: 10.1093/nar/5.1.105.
Abstract
The coding sequence of globin mRNA has been located at or very close to the 3' end of its poly(A)-containing 16S precursor. The 16S RNA was annealed to globin cDNA and the hybrid digested with ribonuclease H. The undigested fragment did not bind to oligo(dT)-cellulose and its size was that expected for the intact 5' portion of the precursor.
摘要
珠蛋白信使核糖核酸（mRNA）的编码序列已定位在其含多聚腺苷酸（poly(A)）的16S前体的3′末端或非常靠近该末端处。将16S核糖核酸（RNA）与珠蛋白互补脱氧核糖核酸（cDNA）退火，并用地核糖核酸酶H消化杂交体。未被消化的片段不与寡聚（dT）纤维素结合，其大小为前体完整5′部分所预期的大小。
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