R4P (Reflection and Research on Resistance to Pesticides) Network, Unité Mixte de Recherche (UMR) Biologie et Gestion des Risques en Agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA), 78850 Thiverval-Grignon, France.
Trends Plant Sci. 2016 Oct;21(10):834-853. doi: 10.1016/j.tplants.2016.06.006. Epub 2016 Jul 27.
Pesticide resistance is a crucial factor to be considered when developing strategies for the minimal use of pesticides while maintaining pesticide efficacy. This goal requires monitoring the emergence and development of resistance to pesticides in crop pests. To this end, various methods for resistance diagnosis have been developed for different groups of pests. This review provides an overview of biological, biochemical, and molecular methods that are currently used to detect and quantify pesticide resistance. The agronomic, technical, and economic advantages and drawbacks of each method are considered. Emerging technologies are also described, with their associated challenges and their potential for the detection of resistance mechanisms likely to be selected by current and future plant protection methods.
当制定最小化使用农药同时保持农药效果的策略时，需要考虑抗药性这一关键因素。这一目标要求监测作物害虫对农药产生和发展的抗药性。为此，已经为不同的害虫群体开发了各种抗性诊断方法。本综述概述了目前用于检测和量化农药抗性的生物、生化和分子方法。考虑了每种方法的农业、技术和经济优势和缺点。还描述了新兴技术，以及它们所面临的挑战及其在检测当前和未来植物保护方法可能选择的抗性机制方面的潜力。
