Aliyu A B, Saleha A A, Jalila A, Zunita Z
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Veterinary Public health laboratory, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
BMC Public Health. 2016 Aug 2;16:699. doi: 10.1186/s12889-016-3377-2.
The significant role of retail poultry meat as an important exposure pathway for the acquisition and transmission of extended spectrum β-lactamase-producing Escherichia coli (ESBL-EC) into the human population warrants understanding concerning those operational practices associated with dissemination of ESBL-EC in poultry meat retailing. Hence, the objective of this study was to determine the prevalence, spatial distribution and potential risk factors associated with the dissemination of ESBL-EC in poultry meat retail at wet-markets in Selangor, Malaysia.
Poultry meat (breast, wing, thigh, and keel) as well as the contact surfaces of weighing scales and cutting boards were sampled to detect ESBL-EC by using culture and disk combination methods and polymerase chain reaction assays. Besides, questionnaire was used to obtain data and information pertaining to those operational practices that may possibly explain the occurrence of ESBL-EC. The data were analysed using logistic regression analysis at 95 % CI.
The overall prevalence of ESBL-EC was 48.8 % (95 % CI, 42 - 55 %). Among the risk factors that were explored, type of countertop, sanitation of the stall environment, source of cleaning water, and type of cutting board were found to be significantly associated with the presence of ESBL-EC.
Thus, in order to prevent or reduce the presence of ESBL-EC and other contaminants at the retail-outlet, there is a need to design a process control system based on the current prevailing practices in order to reduce cross contamination, as well as to improve food safety and consumer health.
零售禽肉作为产超广谱β-内酰胺酶大肠杆菌（ESBL-EC）传入和传播给人群的重要暴露途径，其重要作用使得有必要了解与ESBL-EC在禽肉零售中传播相关的操作规范。因此，本研究的目的是确定马来西亚雪兰莪州湿货市场禽肉零售中ESBL-EC传播的流行率、空间分布及潜在风险因素。
采用培养和纸片联合法及聚合酶链反应检测法，对禽肉（胸脯、翅膀、大腿和龙骨）以及秤和案板的接触面进行采样，以检测ESBL-EC。此外，通过问卷调查获取可能解释ESBL-EC出现的那些操作规范的数据和信息。数据采用95%置信区间的逻辑回归分析。
ESBL-EC的总体流行率为48.8%（95%置信区间，42 - 55%）。在所探究的风险因素中，台面类型、摊位环境卫生、清洁用水来源和案板类型被发现与ESBL-EC的存在显著相关。
因此，为了预防或减少零售点ESBL-EC和其他污染物的存在，有必要根据当前普遍做法设计一个过程控制系统，以减少交叉污染，同时提高食品安全和消费者健康水平。
