Department of Population Health and Reproduction, University of California, Davis, California, USA.
Department of Clinical Pathology, College of Medicine, Mansoura University, Mansoura, Egypt.
Gigascience. 2016 Aug 2;5(1):33. doi: 10.1186/s13742-016-0138-1.
Porites astreoides is a ubiquitous species of coral on modern Caribbean reefs that is resistant to increasing temperatures, overfishing, and other anthropogenic impacts that have threatened most other coral species. We assembled and annotated a transcriptome from this coral using Illumina sequences from three different developmental stages collected over several years: free-swimming larvae, newly settled larvae, and adults (>10 cm in diameter). This resource will aid understanding of coral calcification, larval settlement, and host-symbiont interactions.
A de novo transcriptome for the P. astreoides holobiont (coral plus algal symbiont) was assembled using 594 Mbp of raw Illumina sequencing data generated from five age-specific cDNA libraries. The new transcriptome consists of 867 255 transcript elements with an average length of 685 bases. The isolated P. astreoides assembly consists of 129 718 transcript elements with an average length of 811 bases, and the isolated Symbiodinium sp. assembly had 186 177 transcript elements with an average length of 1105 bases.
This contribution to coral transcriptome data provides a valuable resource for researchers studying the ontogeny of gene expression patterns within both the coral and its dinoflagellate symbiont.
星孔珊瑚是加勒比海域现代珊瑚礁中一种普遍存在的珊瑚物种，它能够抵抗不断升高的温度、过度捕捞以及其他对大多数其他珊瑚物种构成威胁的人为影响。我们利用多年来收集的三个不同发育阶段（自由游动的幼虫、新定居的幼虫和成年珊瑚（直径大于 10 厘米））的 Illumina 序列，组装并注释了这种珊瑚的转录组。这个资源将有助于了解珊瑚钙化、幼虫定殖和宿主共生体相互作用。
使用从五个特定年龄的 cDNA 文库生成的 594 Mbp 的原始 Illumina 测序数据，为星孔珊瑚整体（珊瑚加上藻类共生体）从头组装了一个转录组。新的转录组由 867255 个转录元件组成，平均长度为 685 个碱基。分离的星孔珊瑚组装体由 129718 个转录元件组成，平均长度为 811 个碱基，分离的 Symbiodinium sp. 组装体有 186177 个转录元件，平均长度为 1105 个碱基。
这项对珊瑚转录组数据的贡献为研究珊瑚及其共生鞭毛藻的基因表达模式的个体发生提供了有价值的资源。
