Zhang Jiaying, Li Xiujiang, Xie Jianhua, Huang Zhibing
State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
Foods. 2023 Feb 2;12(3):633. doi: 10.3390/foods12030633.
(1) Background: Medicinal and edible food and traditional Chinese medicine have been used to treat various diseases. However, their safety has not been thoroughly assessed. (2) Methods: An immunochromatographic test strip (ICS) was used for the first time to screen some mycotoxins, including aflatoxin B1 (AFB), zearalenone (ZEN), and T-2 toxin, in medicinal and edible food and traditional Chinese medicine. Antibody/nano-gold particle coupling was used with the prepared ICS, and the pH, monoclonal antibody concentration, and antigen amount were optimized. The extraction sample solution was diluted 10 times with phosphate-buffered saline containing 0.5% Tween-20 and 0.05% sodium dodecyl sulfate to remove the complex matrix in medicinal and edible food. (3) Results: Under optimal conditions, the sensitivities of the developed ICS for AFB, ZEN, and T-2 were 0.5, 5.0, and 5.0 ng/mL, respectively. Among the 30 medicinal and edible food samples tested, two samples (both of sand jujube kernels) were positive, and the results were verified by high-performance liquid chromatography and enzyme-linked immunosorbent assay and were consistent with the ICS test results. (4) Conclusions: The ICS could be used for rapid screening and simultaneous detection of mycotoxins at medicinal and edible food storage facilities.
(1) 背景：药食两用食品和中药已被用于治疗各种疾病。然而，它们的安全性尚未得到全面评估。(2) 方法：首次使用免疫层析试纸条（ICS）对药食两用食品和中药中的一些霉菌毒素进行筛查，包括黄曲霉毒素B1（AFB）、玉米赤霉烯酮（ZEN）和T-2毒素。将抗体/纳米金颗粒偶联物用于制备的ICS，并对pH、单克隆抗体浓度和抗原量进行了优化。提取样品溶液用含有0.5%吐温-20和0.05%十二烷基硫酸钠的磷酸盐缓冲盐水稀释10倍，以去除药食两用食品中的复杂基质。(3) 结果：在最佳条件下，所开发的ICS对AFB、ZEN和T-2的灵敏度分别为0.5、5.0和5.0 ng/mL。在所测试的30个药食两用食品样品中，有两个样品（均为酸枣仁）呈阳性，结果通过高效液相色谱法和酶联免疫吸附测定法进行了验证，与ICS测试结果一致。(4) 结论：ICS可用于药食两用食品储存设施中霉菌毒素的快速筛查和同时检测。
