Lei Wenbin, Li Pei, Han Yongqiang, Gong Shaolong, Yang Lang, Hou Maolin
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China.
Sci Rep. 2016 Aug 5;6:30240. doi: 10.1038/srep30240.
Plant viruses are primarily transmitted by insect vectors and virus infection may influence on the vectors' feeding behaviors. Using an electrical penetration graph, we detected that infection with the Southern rice black-streaked dwarf virus (SRBSDV) in the white-backed planthopper (WBPH) and in rice plants both altered the vector's feeding behavior. When viruliferous WBPH (carrying SRBSDV) were fed on uninfected plants, they spent more time in salivation and phloem sap ingestion than non-viruliferous insects. In comparison with uninfected plants, infected plants showed an arrestant effect on non-viruliferous WBPH for phloem sap ingestion. Differential feeding behaviors were also detected between the WBPH that inoculated or acquired SRBSDV and those that failed to. The WBPH that inoculated SRBSDV exhibited more probing bouts, salivation events and phloem sap ingestion events and longer salivation than those that failed to. The WBPH that acquired SRBSDV were quicker to reach phloem and spent more time in phloem sap ingestion than those that failed to. These behavior alterations in the vector may have adaptive advantages for SRBSDV transmission and spread success because greater salivation by viruliferous vectors on uninfected hosts will promote virus inoculation, whereas more sap ingestion by non-viruliferous vectors on infected hosts will promote virus acquisition.
植物病毒主要通过昆虫媒介传播，病毒感染可能会影响媒介的取食行为。我们使用刺吸电位图谱检测到，感染南方水稻黑条矮缩病毒（SRBSDV）的白背飞虱（WBPH）和水稻植株均改变了媒介的取食行为。当带毒白背飞虱（携带SRBSDV）取食未感染的植株时，它们比无毒昆虫在唾液分泌和韧皮部汁液摄取上花费的时间更多。与未感染的植株相比，感染的植株对无毒白背飞虱的韧皮部汁液摄取表现出抑制作用。在接种或获毒的白背飞虱与未成功接种或获毒的白背飞虱之间也检测到了不同的取食行为。接种SRBSDV的白背飞虱比未接种的表现出更多的刺探次数、唾液分泌事件和韧皮部汁液摄取事件，且唾液分泌时间更长。获毒的白背飞虱比未获毒的更快到达韧皮部，且在韧皮部汁液摄取上花费的时间更多。媒介的这些行为改变可能对SRBSDV的传播和扩散成功具有适应性优势，因为带毒媒介在未感染宿主上更多的唾液分泌将促进病毒接种，而无毒媒介在感染宿主上更多的汁液摄取将促进病毒获取。
