Liu Dandan, Li Zhengxi, Hou Maolin
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Plant Protection, China Agricultural University, Beijing 100193, China.
Insects. 2022 Feb 9;13(2):182. doi: 10.3390/insects13020182.
The southern rice black-streaked dwarf virus (SRBSDV) is transmitted horizontally by in a persistent, propagative manner. Exposure of females to SRBSDV-infected rice plants may trigger transcriptomic changes in the insects, the transcriptomes of females that acquired SRBSDV and those that failed to, as well as females fed on healthy rice plants as control, were sequenced and compared. Nine transcriptomic libraries were constructed, from which a total of 53,084 genes were assembled. Among the genes, 1043 and 2932 were differentially expressed genes (DEGs) in females that acquired SRBSDV and that failed to, in comparison with the control, respectively. Functional enrichment analysis showed that DEGs identified in females exposed to SRBSDV are primarily involved in diverse signaling pathways related to primary metabolism and innate immunity. The DEGs in the females that failed to acquire the virus significantly outnumbered that in the insects that acquired the virus, and the virus exposure activated the humoral and cellular immune responses of the vectors, especially the apoptosis. The key gene in apoptosis encoding caspase 1 was upregulated by SRBSDV exposure, especially in females that failed to acquire the virus. Analysis of caspase 1 activity validated that SRBSDV exposure induced caspase 1 accumulation. Surprisingly, the expression of six female-specific genes was also upregulated by SRBSDV exposure, which was confirmed by RT-qPCR analysis. This study provides evidence to explain the differential virus acquisition at the transcriptome level.
南方水稻黑条矮缩病毒（SRBSDV）以持久、增殖的方式由[昆虫名称]进行水平传播。将[昆虫名称]雌虫暴露于感染SRBSDV的水稻植株可能会引发昆虫的转录组变化，对获取了SRBSDV的雌虫、未获取SRBSDV的雌虫以及作为对照取食健康水稻植株的雌虫的转录组进行了测序和比较。构建了9个转录组文库，从中总共组装了53084个基因。在这些基因中，与对照相比，获取了SRBSDV的[昆虫名称]雌虫和未获取SRBSDV的[昆虫名称]雌虫中分别有1043个和2932个差异表达基因（DEG）。功能富集分析表明，暴露于SRBSDV的[昆虫名称]雌虫中鉴定出的DEG主要参与与初级代谢和先天免疫相关的多种信号通路。未获取病毒的[昆虫名称]雌虫中的DEG数量显著多于获取了病毒的昆虫中的DEG数量，并且病毒暴露激活了介体的体液免疫和细胞免疫反应，尤其是凋亡。编码半胱天冬酶1的凋亡关键基因在暴露于SRBSDV时上调，尤其是在未获取病毒的[昆虫名称]雌虫中。对半胱天冬酶1活性的分析证实，SRBSDV暴露诱导了半胱天冬酶1的积累。令人惊讶的是，SRBSDV暴露还上调了6个雌性特异性基因的表达，这通过RT-qPCR分析得到了证实。本研究为在转录组水平解释病毒获取差异提供了证据。
