机械刷牙联合不同假牙清洁剂对降低丙烯酸树脂上多物种生物膜活力的影响。
Effect of mechanical toothbrushing combined with different denture cleansers in reducing the viability of a multispecies biofilm on acrylic resins.
作者信息
Panariello Beatriz Helena Dias, Izumida Fernanda Emiko, Moffa Eduardo Buozi, Pavarina Ana Claudia, Jorge Janaina Habib, Giampaolo Eunive Teresinha
出版信息
Am J Dent. 2016 Jun;29(3):154-60.
PURPOSE
To investigate the efficacy of immersion and brushing with different cleansing agents in reducing the viability of multispecies biofilm on acrylic resins.
METHODS
Lucitone 550 (L) and Tokuyama Rebase Fast II (T) specimens (10 x 2 mm) were prepared, sterilized, and inoculated with a suspension of Candida albicans, Candida glabrata, and Streptococcus mutans. Specimens were incubated for 48 hours at 37 degrees C for biofilm formation. Then, they were divided into groups (n = 12) and subjected to brushing or immersion for 10 seconds in distilled water (W), 0.2% peracetic acid-Sterilife (Ac), 1% chlorhexidine digluconate (CHX), 1:1 water/dentifrice solution (D), 1% sodiumhypochlorite (NaOCl), and sodium perborate/Corega Tabs (Pb). Viable microorganisms were evaluated by the XTT assay and colony counts (cfu/mL). Data were performed by ANOVA and Tukey test with 5% significance level.
RESULTS
The multispecies biofilm on L and T were killed by brushing or immersion in Ac, CHX, and NaOCl for only 10 seconds.
目的
研究使用不同清洁剂浸泡和刷牙对降低丙烯酸树脂上多菌种生物膜活力的效果。
方法
制备Lucitone 550（L）和德山快速重衬II型（T）标本（10×2毫米），进行灭菌，并用白色念珠菌、光滑念珠菌和变形链球菌的悬浮液接种。标本在37℃下孵育48小时以形成生物膜。然后，将它们分成几组（n = 12），并在蒸馏水（W）、0.2%过氧乙酸-Sterilife（Ac）、1%葡萄糖酸氯己定（CHX）、1:1水/牙膏溶液（D）、1%次氯酸钠（NaOCl）和过硼酸钠/Corega Tabs（Pb）中刷牙或浸泡10秒。通过XTT试验和菌落计数（cfu/mL）评估存活微生物。数据采用方差分析和Tukey检验，显著性水平为5%。
结果
仅通过在Ac、CHX和NaOCl中刷牙或浸泡10秒，L和T上的多菌种生物膜就被杀死。
Zotero 插件