Liu Xi-Ping, Ming Hai-Xia, Li Pei-Qing
Basic Medical College, Gansu University of Chinese Medicine Lanzhou 730000, China.
Am J Transl Res. 2016 Jul 15;8(7):2937-46. eCollection 2016.
The study aimed to simulate the microenvironment of gastric cancer to promote the malignant transformation of bone marrow mesenchymal stem cells (BMSCs) and further evaluate the effect of Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions on BMSCs.
Transwell co-culture was performed on the human gastric cancer cell strains BGC-823 and BMSCs to simulate the microenvironment of gastric cancer. The drug-containing serum prepared by Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions was used, and its influence on BMSCs with malignant transformation was observed.
BMSCs were harvested successfully from the rat bone marrow, and flow cytometer identification indicated that CD44+/CD34- cells accounted for 70.64%. The co-culture of BGC-823 cells can induce malignant transformation of BMSCs. And the drug-containing serum can induce G2 phase arrest, inhibit cell proliferation, simultaneously inhibit TERT and c-myc expression, lower the cellular ability of chemotactic migration, inhibit the tumor-forming ability of BGC-823 in nude rats and promote the tumor apoptosis.
The effective components of Pinelliae Decoction for Purging Stomach-Fire in gastric cancer treatment are pinelliae and dried ginger, and the main acting mechanism is to inhibit tumor cell proliferation and chemotactic migration and promote apoptosis.
本研究旨在模拟胃癌微环境以促进骨髓间充质干细胞（BMSCs）的恶性转化，并进一步评估泻心汤及其拆方对BMSCs的作用。
采用人胃癌细胞株BGC-823与BMSCs进行Transwell共培养以模拟胃癌微环境。使用泻心汤及其拆方制备含药血清，观察其对发生恶性转化的BMSCs的影响。
成功从大鼠骨髓中获取BMSCs，流式细胞仪鉴定显示CD44+/CD34-细胞占70.64%。BGC-823细胞共培养可诱导BMSCs发生恶性转化。含药血清可诱导细胞G2期阻滞，抑制细胞增殖，同时抑制TERT和c-myc表达，降低细胞趋化迁移能力，抑制BGC-823在裸鼠体内的成瘤能力并促进肿瘤凋亡。
泻心汤治疗胃癌的有效成分为半夏和干姜，主要作用机制为抑制肿瘤细胞增殖和趋化迁移并促进凋亡。
