Kračun-Kolarević Margareta, Kolarević Stoimir, Atanacković Ana, Marković Vanja, Gačić Zoran, Paunović Momir, Vuković-Gačić Branka
Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despota Stefana, 11060 Belgrade, Serbia.
Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
Water Air Soil Pollut. 2015;226:242. doi: 10.1007/s11270-015-2511-6. Epub 2015 Jul 7.
Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl) was evaluated in , cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 μM), ET (0.004. 0.04, 0.4 and 4 μM) and CdCl (0.004, 0.04, 0.4, 4 and 40 μM). Mortality of worms was observed only for CdCl (4 and 40 μM). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 μM), ET (4 μM) and CdCl (0.4 and 4 μM). All tested substances induced significant increase of DNA damage except 0.004 μM of ET. being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 μM induced DNA damage is similar to ones detected in wastewaters.
通过碱性单细胞凝胶电泳（彗星试验），对世界性颤蚓物种进行了5-氟尿嘧啶（5-FU）、依托泊苷（ET）和氯化镉（CdCl）的遗传毒性评估。在仅含水的短期（96小时）试验中，将50个个体的组暴露于5-FU（0.004、0.04、0.4、4和40μM）、ET（0.004、0.04、0.4和4μM）和CdCl（0.004、0.04、0.4、4和40μM）。仅在CdCl（4和40μM）处理组中观察到蠕虫死亡。在5-FU（0.4、4和40μM）、ET（4μM）和CdCl（0.4和4μM）处理组中检测到细胞活力低于70%。除0.004μM的ET外，所有测试物质均导致DNA损伤显著增加。 对所有测试物质敏感表明它可用于生态遗传毒理学研究。应关注细胞抑制剂，尤其是5-FU，因为0.004μM浓度诱导的DNA损伤与在废水中检测到的损伤相似。
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