Shen Clara, Kolanowski Jacek L, Tran Carmen M-N, Kaur Amandeep, Akerfeldt Mia C, Rahme Matthew S, Hambley Trevor W, New Elizabeth J
School of Chemistry, The University of Sydney, NSW 2006, Australia.
Metallomics. 2016 Sep 1;8(9):915-9. doi: 10.1039/c6mt00083e. Epub 2016 Aug 23.
Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.
铜在细胞功能调节、防御和生长过程中发挥着关键作用。在此，我们展示了InCCu1，一种用于检测线粒体铜的比率荧光传感器，在存在Cu(i)的情况下，其发射光会从红色变为蓝色。通过在显微镜和流式细胞术中使用该探针，我们发现顺铂处理的细胞在线粒体中积累铜的能力受损。
