Muñoz Calvo B
Med Clin (Barc). 1989 May 13;92(18):681-3.
Three patients with an histological, clinical and immunological diagnosis of chronic autoimmune (lupoid) hepatitis (CAH) and negative hepatitis B virus (HBV) markers were evaluated. The hepatic tissue of these patients, which was obtained through transjugular hepatic biopsy, was investigated with radioactive DNA probes of the previously cloned HBV and hybridized with the DNA from the mentioned tissues. In two of the three samples, viral nucleotide sequences were detected in the nuclei of the hepatocytes. Their appearance was consistent either with a free state or with the presence of integrated concatomeres. Although these findings require further evaluation in larger series, they suggest the possibility that certain proteins encoded or modified by the HBV DNA might represent a hidden antigenic stimulus in an autoimmune disease such as CAH, even in the absence of serological markers of HBV infection.
对3例经组织学、临床和免疫学诊断为慢性自身免疫性（狼疮样）肝炎（CAH）且乙肝病毒（HBV）标志物阴性的患者进行了评估。通过经颈静脉肝活检获取这些患者的肝组织，用先前克隆的HBV放射性DNA探针进行研究，并与上述组织的DNA杂交。在三个样本中的两个样本中，在肝细胞核中检测到病毒核苷酸序列。它们的出现与游离状态或整合串联体的存在一致。尽管这些发现需要在更大系列中进一步评估，但它们提示了一种可能性，即即使在没有HBV感染血清学标志物的情况下，由HBV DNA编码或修饰的某些蛋白质可能在诸如CAH的自身免疫性疾病中代表一种隐藏的抗原刺激。
