Minichiello M M, Albert D M, Kolodny N H, Lee M S, Craft J L
Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114.
Magn Reson Med. 1989 Apr;10(1):96-107. doi: 10.1002/mrm.1910100109.
A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage-dependent cells grew to tissue-like density.
通过采用Vitafiber I柱系统（密理博公司），已开发出一种用于人葡萄膜黑色素瘤代谢的31P核磁共振研究的灌注培养系统。在长达10周的时间里每周收集的31P核磁共振谱显示，随着贴壁依赖性细胞生长至组织样密度，磷代谢物水平不断升高。
