Kolodny N H, Albert D M, Epstein J, Ruzzo M, Sprengnether M
Am J Ophthalmol. 1985 Jul 15;100(1):38-44. doi: 10.1016/s0002-9394(14)74980-3.
We performed experiments to determine the potential usefulness of nuclear magnetic resonance spectra in the diagnosis and follow-up of ocular melanoma. High-resolution phosphorus 31 nuclear magnetic resonance spectra at 109.3 MHz were obtained for human uveal melanoma, Greene hamster melanoma, and normal human diploid fibroblast cells. Phosphate metabolites were identified and their concentrations were shown to vary among the different cell lines. Uveal melanoma cells contain unusually high concentrations of the phospholipid metabolite phosphorylcholine and the phosphodiesters glycerol 3-phosphoryl choline and glycerol 3-phosphoryl ethanolamine. Baseline data are thus provided for studies of the effect of various treatment modalities on uveal melanoma. These initial results suggest that the data provided by high-resolution phosphorus 31 nuclear magnetic resonance spectra can provide useful diagnostic and follow-up data with respect to ocular melanoma.
我们进行了实验，以确定核磁共振光谱在眼黑色素瘤诊断和随访中的潜在用途。获得了109.3兆赫下人类葡萄膜黑色素瘤、格林仓鼠黑色素瘤和正常人二倍体成纤维细胞的高分辨率磷31核磁共振光谱。鉴定了磷酸盐代谢物，其浓度在不同细胞系中有所不同。葡萄膜黑色素瘤细胞含有异常高浓度的磷脂代谢物磷酸胆碱以及磷酸二酯甘油3 - 磷酸胆碱和甘油3 - 磷酸乙醇胺。因此，为研究各种治疗方式对葡萄膜黑色素瘤的影响提供了基线数据。这些初步结果表明，高分辨率磷31核磁共振光谱提供的数据可以为眼黑色素瘤提供有用的诊断和随访数据。
