Stevenson Emma C, Major Giles A, Spiller Robin C, Kuehne Sarah A, Minton Nigel P
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
NIHR Biomedical Research Unit in Gastrointestinal and Liver Diseases at Nottingham University Hospitals NHS Trust and The University of Nottingham, Nottingham, United Kingdom.
J Clin Microbiol. 2016 Nov;54(11):2689-2694. doi: 10.1128/JCM.01025-16. Epub 2016 Aug 24.
Clostridium difficile (Peptoclostridium difficile) is a common health care-associated infection with a disproportionately high incidence in elderly patients. Disease symptoms range from mild diarrhea to life-threatening pseudomembranous colitis. Around 20% of patients may suffer recurrent disease, which often requires rehospitalization of patients. C. difficile was isolated from stool samples from a patient with two recurrent C. difficile infections. PCR ribotyping, whole-genome sequencing, and phenotypic assays were used to characterize these isolates. Genotypic and phenotypic screening of C. difficile isolates revealed multiple PCR ribotypes present and the emergence of rifamycin resistance during the infection cycle. Understanding both the clinical and bacterial factors that contribute to the course of recurrent infection could inform strategies to reduce recurrence. (This study has been registered at ClinicalTrials.gov under registration no. NCT01670149.).
艰难梭菌（难辨梭状芽孢杆菌）是一种常见的医疗保健相关感染，在老年患者中的发病率高得不成比例。疾病症状从轻度腹泻到危及生命的伪膜性结肠炎不等。约20%的患者可能会复发疾病，这通常需要患者再次住院。从一名患有两次复发性艰难梭菌感染的患者的粪便样本中分离出艰难梭菌。使用PCR核糖体分型、全基因组测序和表型分析来鉴定这些分离株。对艰难梭菌分离株的基因型和表型筛选显示存在多种PCR核糖体分型，并且在感染周期中出现了利福霉素耐药性。了解导致复发性感染病程的临床和细菌因素可为减少复发的策略提供依据。（本研究已在ClinicalTrials.gov上注册，注册号为NCT01670149。）
