Egashira Nobuaki, Iba Hikari, Kuwano Haruna, Kawanaka Rikako, Nagao Masaki, Moriyama Hiroshi, Watanabe Takuya, Kubota Kaori, Katsurabayashi Shutaro, Iwasaki Katsunori
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan; Department of Pharmacy, Kyushu University Hospital, Fukuoka 812-8582, Japan.
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
J Pharmacol Sci. 2016 Aug;131(4):279-83. doi: 10.1016/j.jphs.2016.07.007. Epub 2016 Jul 30.
In the present study, we investigated the effect of kamishoyosan (KSS) on conditioned fear-induced freezing in ovariectomized (OVX) rats. Socially isolated OVX rats showed the longest freezing time among the following four groups: group-housed sham-operated (Sham), isolated Sham, group-housed OVX, and isolated OVX rats. Repeated oral administration of KSS (30-300 mg/kg) reduced conditioned fear-induced freezing in socially isolated OVX rats. The reduction of freezing by KSS was reversed by flumazenil (3 mg/kg) and bicuculline (3 mg/kg). These findings suggest that the GABAA-benzodiazepine receptor complex is involved in the anxiolytic effect of KSS in socially isolated OVX rats.
在本研究中，我们调查了加味逍遥散（KSS）对去卵巢（OVX）大鼠条件性恐惧诱导的僵住反应的影响。在以下四组大鼠中，社会隔离的OVX大鼠表现出最长的僵住时间：群居假手术（Sham）组、隔离假手术组、群居OVX组和隔离OVX组大鼠。对社会隔离的OVX大鼠重复口服KSS（30 - 300毫克/千克）可减少条件性恐惧诱导的僵住反应。氟马西尼（3毫克/千克）和荷包牡丹碱（3毫克/千克）可逆转KSS对僵住反应的降低作用。这些发现表明，GABAA-苯二氮䓬受体复合物参与了KSS对社会隔离的OVX大鼠的抗焦虑作用。
